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Here, the role of fibroblasts \u2013 a major component of the dermal microenvironment \u2013 on the migratory capacity of monocyte\u2010derived <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> (Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>) was investigated in a 3D collagen I matrix. Indeed, dermal fibroblasts supported the migration of pre\u2010activated Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> through a 3D collagen I matrix. Activation of human Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> resulted in the release of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u03b1 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 that in turn stimulated <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 (human collagenase) and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content><jats:sub>2<\/jats:sub> secretion by human dermal fibroblasts. Transmigration assays confirmed the importance of fibroblast\u2010derived <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content><jats:sub>2<\/jats:sub> for the migration of Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> through a 3D collagen I matrix. Finally, in mice initiation of inflammation by induction of an irritant contact dermatitis or a psoriasis\u2010like skin inflammation, the expression of the <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content><jats:sub>2<\/jats:sub> generating cox\u20102 and the mouse collagen I degrading enzyme matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>)\u201013 was strongly up\u2010regulated. Our study indicates that Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> are able to instruct dermal fibroblasts resulting in enhanced migratory capability of Mo<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>, thus highlighting the role of a crosstalk of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> with their stromal microenvironment for the control of cutaneous immune responses.<\/jats:p>","DOI":"10.1111\/exd.12722","type":"journal-article","created":{"date-parts":[[2015,4,11]],"date-time":"2015-04-11T15:42:49Z","timestamp":1428766969000},"page":"598-604","source":"Crossref","is-referenced-by-count":23,"title":["Fibroblasts support migration of monocyte\u2010derived dendritic cells by secretion of <scp>PGE<\/scp><sub>2<\/sub> and <scp>MMP<\/scp>\u20101"],"prefix":"10.1111","volume":"24","author":[{"given":"Anja","family":"Saalbach","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venerology and 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