{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:43:46Z","timestamp":1790808226545,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oligosaccharide modification by <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content><\/jats:italic>\u2010acetylglucosaminyltransferase\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>), which catalyses the formation of <jats:italic>\u03b2<\/jats:italic>1,6 <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lc<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>c (<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010acetylglucosamine) branches on <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content><\/jats:italic>\u2010glycans, is associated with various pathologies, such as cancer metastasis, multiple sclerosis and liver fibrosis. In this study, we demonstrated the involvement of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> in the pathophysiology of scleroderma. High expression of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> was observed in infiltrating cells in skin section samples from systemic and localized patients with scleroderma. Most of the infiltrating cells were <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells and macrophages, most of which were <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>2 macrophages. To determine the role of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> in scleroderma, we next investigated skin sclerosis in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> knockout (<jats:italic><jats:styled-content style=\"fixed-case\">MGAT<\/jats:styled-content>5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup>) mice. Expression of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> was also elevated in bleomycin (<jats:styled-content style=\"fixed-case\">BLM<\/jats:styled-content>)\u2010injected sclerotic skin, and <jats:italic><jats:styled-content style=\"fixed-case\">MGAT<\/jats:styled-content>5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice were resistant to <jats:styled-content style=\"fixed-case\">BLM<\/jats:styled-content>\u2010induced skin sclerosis with reduced collagen type 1 <jats:italic>\u03b1<\/jats:italic>1 content, suggesting the biological significance of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> in skin sclerosis. Furthermore, the number of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>2 macrophages and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3\u2010positive <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells in <jats:styled-content style=\"fixed-case\">BLM<\/jats:styled-content>\u2010induced skin sclerosis was significantly fewer in <jats:italic><jats:styled-content style=\"fixed-case\">MGAT<\/jats:styled-content>5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice. In bone marrow\u2010derived macrophages (<jats:styled-content style=\"fixed-case\">BMDM<\/jats:styled-content>s), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104\u2010induced expressions of <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>izz1 and <jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>m1 were significantly reduced in <jats:italic><jats:styled-content style=\"fixed-case\">MGAT<\/jats:styled-content>5<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice\u2010derived <jats:styled-content style=\"fixed-case\">BMDM<\/jats:styled-content>s. Taken together, these results suggest the induction of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>n<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content> in skin sclerosis progression is possibly dependent on increased numbers of <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>2 macrophages in the skin, which are important for tissue fibrosis and remodelling.<\/jats:p>","DOI":"10.1111\/exd.12730","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T14:42:14Z","timestamp":1429108934000},"page":"585-590","source":"Crossref","is-referenced-by-count":16,"title":["Oligosaccharide modification by <i><scp>N<\/scp><\/i>\u2010acetylglucosaminyltransferase\u2010<scp>V<\/scp> in macrophages are involved in pathogenesis of bleomycin\u2010induced scleroderma"],"prefix":"10.1111","volume":"24","author":[{"given":"Arisa","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"},{"name":"Department of Molecular Biochemistry and Clinical Investigation Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuki","family":"Yutani","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"},{"name":"Department of Molecular Biochemistry and Clinical Investigation Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Terao","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"},{"name":"Department of Molecular Biochemistry and Clinical Investigation Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saori","family":"Itoi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Murota","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biochemistry and Clinical Investigation Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Katayama","sequence":"additional","affiliation":[{"name":"Department of Dermatology Graduate School of Medicine Osaka University Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.57.070188.004033"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4165(99)00167-1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000151709"},{"key":"e_1_2_9_5_1","first-page":"1772","volume":"6","author":"Murata K","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/73163"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.220376"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01515.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840220634"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2012.45.10.190"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cws012"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/35055582"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.12.7200"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1333"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.10.026"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.01.003"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.279554"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.10-OA-0283"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(88)70070-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12237"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12409"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12256"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00973.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2008.00828.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33746-3"},{"key":"e_1_2_9_26_1","first-page":"1202","volume":"19","author":"Ishikawa O","year":"1992","journal-title":"J Rheumatol"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI59643"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903356"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-010-1691-z"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-012-1972-x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-012-0297-8"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24432"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000082331"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.11.024"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12730","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:09:30Z","timestamp":1695503370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,26]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/exd.12730"],"URL":"https:\/\/doi.org\/10.1111\/exd.12730","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,26]]}}}