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We compared the effects of two excipients, sodium caprate (C10) and a self\u2010emulsifying excipient Labrasol composed of a mixture of caprylocaproyl polyoxyl\u20108 glycerides, both applied to emerged reconstructed human epidermis either \u2018systemically\u2019, that is by addition to the culture medium, or topically. During the \u2018systemic\u2019 application, which produced cytoplasmic translocation of occludin and leakage of the biotin marker into the lower <jats:italic>stratum corneum<\/jats:italic>, the decrease in the trans\u2010epithelial electrical resistance (<jats:styled-content style=\"fixed-case\">TEER<\/jats:styled-content>) was less abrupt with Labrasol when compared with C10, even though both excipients produced comparable final effects over time. With topical Labrasol, a significant <jats:styled-content style=\"fixed-case\">TEER<\/jats:styled-content> decrease was obtained with 5 times the \u2018systemic\u2019 concentrations. Topical application of C10 also resulted in the loss of the barrier function measured with <jats:styled-content style=\"fixed-case\">TEER<\/jats:styled-content> but had dramatic deleterious effects on the tissue morphology observed with light and electron microscopy. Our study demonstrates the potential value of Labrasol as an enhancer of bioavailability of molecules applied through the transcutaneous route. Our results suggest modulation of the epidermal <jats:styled-content style=\"fixed-case\">TJ<\/jats:styled-content>s by both compounds. Even though the C10 action was at least partly due to overall cell damage and despite the fact that the decrease in <jats:styled-content style=\"fixed-case\">TEER<\/jats:styled-content> after topical application was apparently related to the permeabilization of the primary barrier of the <jats:italic>stratum corneum<\/jats:italic> in the first place.<\/jats:p>","DOI":"10.1111\/exd.12750","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T06:11:42Z","timestamp":1431065502000},"page":"686-691","source":"Crossref","is-referenced-by-count":36,"title":["Modulation of transepithelial electric resistance (<scp>TEER<\/scp>) in reconstructed human epidermis by excipients known to permeate intestinal tight junctions"],"prefix":"10.1111","volume":"24","author":[{"given":"Rawad","family":"Abdayem","sequence":"first","affiliation":[{"name":"Fundamental, clinical and therapeutic aspects of the skin barrier function Universit\u00e9 Lyon 1 Lyon 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