{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:43:40Z","timestamp":1770911020767,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T00:00:00Z","timestamp":1443139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mucin\u2010like transmembrane protein podoplanin (<jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content>) is prominently represented in tumor\u2010associated gene expression signatures of numerous types of cancer including squamous cell carcinoma, and gain\u2010of\u2010function and knockdown approaches in tissue culture strongly suggested an important role of <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> in cell proliferation, migration and adhesion. <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> is absent during epidermal homeostasis but is highly expressed in basal keratinocytes during cutaneous wound healing. Enhanced motility of immortalized keratinocytes upon ectopic <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> overexpression argues for wound healing defects upon podoplanin deficiency in keratinocytes; however, <jats:italic>in\u00a0vivo<\/jats:italic> data that unequivocally define the impact of <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> by functional studies in a physiologically relevant system are still missing. Here, we have applied an <jats:italic>in\u00a0vivo<\/jats:italic> loss\u2010of\u2010function approach by generating a novel transgenic mouse line with keratinocyte\u2010specific podoplanin deficiency. Performing cutaneous full\u2010thickness excisional wounds to examine re\u2010epithelialization capacity, unexpectedly, no defects were observed in wound healing properties of mutant mice. Similarly, <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content>\u2010deficient primary keratinocytes showed no impairment in migration, adhesion or proliferation. Thus, <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> function is not rate\u2010limiting for re\u2010epithelialization but may be functionally compensated by an as yet unknown protein. Our data also call for <jats:italic>in\u00a0vivo<\/jats:italic> functional studies on <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> in settings of skin tumor development and progression to clarify <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content>'s role in skin pathology.<\/jats:p>","DOI":"10.1111\/exd.12781","type":"journal-article","created":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T11:26:20Z","timestamp":1435577180000},"page":"785-787","source":"Crossref","is-referenced-by-count":10,"title":["Epithelial deletion of podoplanin is dispensable for re\u2010epithelialization of skin wounds"],"prefix":"10.1111","volume":"24","author":[{"given":"Sebastian","family":"Baars","sequence":"first","affiliation":[{"name":"Division of Signal Transduction and Growth Control DKFZ\u2010ZMBH Alliance German Cancer Research Center (DKFZ) Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Bauer","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction and Growth Control DKFZ\u2010ZMBH Alliance German Cancer Research Center (DKFZ) Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibylle","family":"Szabowski","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction and Growth Control DKFZ\u2010ZMBH Alliance German Cancer Research Center (DKFZ) Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Hartenstein","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction and Growth Control DKFZ\u2010ZMBH Alliance German Cancer Research Center (DKFZ) Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Angel","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction and Growth Control DKFZ\u2010ZMBH Alliance German Cancer Research Center (DKFZ) Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00283"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199709)20:1<10::AID-MC3>3.0.CO;2-M"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg342"},{"issue":"24","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"4601","DOI":"10.1242\/jcs.112.24.4601","volume":"112","author":"Scholl F G","year":"1999","journal-title":"J Cell Sci"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.03.010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03218"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.078170"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0299"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.388"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.12781","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.12781","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T06:29:14Z","timestamp":1695536954000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.12781"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,25]]},"references-count":9,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/exd.12781"],"URL":"https:\/\/doi.org\/10.1111\/exd.12781","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,25]]}}}