{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T01:56:47Z","timestamp":1785549407426,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25461682"],"award-info":[{"award-number":["25461682"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26461677"],"award-info":[{"award-number":["26461677"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mycosis fungoides (<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>) starts as an indolent disease, progresses from a patch stage to confluent plaques and ultimately develops skin tumors. Tumor\u2010associated macrophages (<jats:styled-content style=\"fixed-case\">TAM<\/jats:styled-content>s) play roles in maintaining the tumor microenvironment in <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>. The purpose of this study was to elucidate the involvement of <jats:styled-content style=\"fixed-case\">TAM<\/jats:styled-content>s in the lesional skin of different stages of <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>. First, we immunohistologically examined the percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163<jats:sup>+<\/jats:sup> macrophages and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>206<jats:sup>+<\/jats:sup> cells, as well as the levels of periostin and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 in cancer stroma. The percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>206<jats:sup>+<\/jats:sup> cells increased in parallel with tumor progression, while there was no significant difference in the percentage of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163<jats:sup>+<\/jats:sup> cells. Periostin was prominent in the stromal area at the patch and plaque stages but decreased at the tumor stage. In contrast, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 was prominently stained at both plaque and tumor stages. To further elucidate the molecular mechanisms of the effects of these stromal factors on <jats:styled-content style=\"fixed-case\">TAM<\/jats:styled-content>s, we examined their effects on <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in monocyte\u2010derived macrophages <jats:italic>in vitro<\/jats:italic>. Based on microarray analysis and gene ontology, we examined a series of chemokines and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s whose expression was strongly connected with periostin stimulation. The <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> microarray results were verified in M2 macrophages using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. We further examined the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of these chemokines and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s in the presence of periostin and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 to simulate the advanced stages of <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> and validated their protein expression by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Our present report suggests possible roles of periostin on <jats:styled-content style=\"fixed-case\">TAM<\/jats:styled-content>s in establishing the tumor microenvironment in MF.<\/jats:p>","DOI":"10.1111\/exd.12873","type":"journal-article","created":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T16:09:09Z","timestamp":1444147749000},"page":"107-112","source":"Crossref","is-referenced-by-count":68,"title":["The possible interaction between periostin expressed by cancer stroma and tumor\u2010associated macrophages in developing mycosis fungoides"],"prefix":"10.1111","volume":"25","author":[{"given":"Sadanori","family":"Furudate","sequence":"first","affiliation":[{"name":"Department of Dermatology Tohoku University Graduate School of Medicine Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tohoku University Graduate School of Medicine Sendai 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