{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:34:23Z","timestamp":1789893263653,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development","award":["1BX001680-01"],"award-info":[{"award-number":["1BX001680-01"]}]},{"DOI":"10.13039\/100015515","name":"Carver College of Medicine","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015515","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011343","name":"Holden Comprehensive Cancer Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011343","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Iowa City Veteran's Administration Medical Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bullous pemphigoid (<jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>) is an autoimmune blistering disease characterized by antibodies (IgG and IgE) targeting cell\u2010substrate adhesion proteins. A variety of BP models suggest that autoantibody\u2010dependent neutrophil degranulation is essential for blister formation. However, lesional biopsies reveal a predominance of eosinophils and few neutrophils. Our goal was to evaluate the role of antibodies and complement in eosinophil localization, degranulation and split formation at the dermo\u2010epidermal junction (<jats:styled-content style=\"fixed-case\">DEJ<\/jats:styled-content>) utilizing a human skin cryosection model of BP paired with a human eosinophilic cell line, 15HL\u201060. Expression of receptors for IgG (Fc\u03b3RII), IgE (Fc\u03b5RI) and complement (CR1 and CR3) was confirmed on 15HL\u201060 cells using flow cytometry. 15HL\u201060 expression of granule protein [eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (<jats:styled-content style=\"fixed-case\">EPO<\/jats:styled-content>)] m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and their degranulation <jats:italic>in vitro<\/jats:italic> was confirmed using RT\u2010PCR and ELISA, respectively. For cryosection experiments, BP or control sera or IgG and IgE antibodies purified from BP sera were utilized in combination with 15HL\u201060 cells \u00b1 fresh complement. Both BP serum and fresh complement were required for localization of 15\u2010HL60 cells to the DEJ. Interestingly, eosinophil localization to the DEJ was dependent on IgG, but not IgE, and complement. However, no subepidermal split was observed. Additionally, the 15HL\u201060 cells did not degranulate under any experimental conditions and direct application of cell lysate to cryosections did not result in a split. Our observation that eosinophil localization to the DEJ is dependent on IgG mediated complement fixation provides additional insight into the sequence of events during the development of BP lesions.<\/jats:p>","DOI":"10.1111\/exd.12883","type":"journal-article","created":{"date-parts":[[2015,10,17]],"date-time":"2015-10-17T15:28:42Z","timestamp":1445095722000},"page":"50-55","source":"Crossref","is-referenced-by-count":29,"title":["Eosinophil localization to the basement membrane zone is autoantibody\u2010 and complement\u2010dependent in a human cryosection model of bullous pemphigoid"],"prefix":"10.1111","volume":"25","author":[{"given":"Kelly N.","family":"Messingham","sequence":"first","affiliation":[{"name":"Department of Dermatology University of Iowa Iowa City IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey W.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Iowa Iowa City IA 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