{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:42:57Z","timestamp":1790635377544,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T00:00:00Z","timestamp":1452556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japanese Ministry of Health, Labour and Welfare"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"Abstract<\/jats:title>\n \n Long non\u2010coding\n RNA<\/jats:styled-content>\n s (lnc\n RNA<\/jats:styled-content>\n s) are thought to have various functions other than\n RNA<\/jats:styled-content>\n silencing. We tried to evaluate the expression of lnc\n RNA<\/jats:styled-content>\n s in patients with systemic sclerosis (\n SS<\/jats:styled-content>\n c) and determined whether lnc\n RNA<\/jats:styled-content>\n s controls collagen expression in dermal fibroblasts. lnc\n RNA<\/jats:styled-content>\n expression was determined by real\u2010time\n PCR<\/jats:styled-content>\n and\n in situ<\/jats:italic>\n hybridization. Protein and\n mRNA<\/jats:styled-content>\n levels of collagen were analysed using immunoblotting and real\u2010time\n PCR<\/jats:styled-content>\n . We found\n TSIX<\/jats:styled-content>\n , one of the lnc\n RNA<\/jats:styled-content>\n s, was overexpressed in\n SS<\/jats:styled-content>\n c dermal fibroblasts both\n in vivo<\/jats:italic>\n and\n in vitro<\/jats:italic>\n , which was inhibited by the transfection of transforming growth factor (\n TGF<\/jats:styled-content>\n )\u2010\u03b21 si\n RNA<\/jats:styled-content>\n .\n TSIX<\/jats:styled-content>\n si\n RNA<\/jats:styled-content>\n reduced the\n mRNA<\/jats:styled-content>\n expression of type I collagen in normal and\n SS<\/jats:styled-content>\n c dermal fibroblasts, but not the levels of major disease\u2010related cytokines. In addition,\n TSIX<\/jats:styled-content>\n si\n RNA<\/jats:styled-content>\n significantly reduced type I collagen\n mRNA<\/jats:styled-content>\n stability, but not protein half\u2010lives. Furthermore, we first investigated serum lnc\n RNA<\/jats:styled-content>\n levels in patients with\n SS<\/jats:styled-content>\n c, and serum\n TSIX<\/jats:styled-content>\n levels were significantly increased in\n SS<\/jats:styled-content>\n c patients.\n TSIX<\/jats:styled-content>\n is a new regulator of collagen expression which stabilizes the collagen\n mRNA<\/jats:styled-content>\n . The upregulation of\n TSIX<\/jats:styled-content>\n seen in\n SS<\/jats:styled-content>\n c fibroblasts may result from activated endogenous\n TGF<\/jats:styled-content>\n \u2010\u03b2 signalling and may play a role in the constitutive upregulation of collagen in these cells. Further studies on the regulatory mechanism of tissue fibrosis by lnc\n RNA<\/jats:styled-content>\n s in\n SS<\/jats:styled-content>\n c skin lead to a better understanding of the pathogenesis, new diagnostic methods by their serum levels and new therapeutic approaches using siRNAs.\n <\/jats:p>","DOI":"10.1111\/exd.12900","type":"journal-article","created":{"date-parts":[[2015,11,14]],"date-time":"2015-11-14T03:22:36Z","timestamp":1447471356000},"page":"131-136","source":"Crossref","is-referenced-by-count":71,"title":["Long non\u2010coding\n RNA TSIX<\/scp>\n is upregulated in scleroderma dermal fibroblasts and controls collagen\n mRNA<\/scp>\n stabilization"],"prefix":"10.1111","volume":"25","author":[{"given":"Zhongzhi","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University Kumamoto 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