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Recent evidence suggests that dimethylfumarate (<jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content>), an antipsoriatic agent, might have antitumorigenic and antilymphangiogenic properties. To prove this assumption, we performed proliferation and functional assays with primary human dermal lymphendothelial cells (<jats:styled-content style=\"fixed-case\">DLEC<\/jats:styled-content>). We could demonstrated that <jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content> suppresses <jats:styled-content style=\"fixed-case\">DLEC<\/jats:styled-content> proliferation and formation of capillary\u2010like structures. Underlying apoptotic mechanisms could be ruled out. Cell cycle analysis demonstrated a pronounced G1\u2010arrest. Further evaluations revealed increases in p21 expression. In addition, <jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content> suppressed Cyclin D1 and Cyclin A expression in a concentration\u2010dependent manner. p21 knockdown experiments demonstrated a p21\u2010dependent mechanism of regulation. Further analysis showed an increased p21 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression after <jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content> treatment. This transcriptional regulation was enforced by post\u2010transcriptional and post\u2010translational mechanisms. In addition, we could demonstrate that the combination of a proteasomal inhibitor and <jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content> superinduced the p21 expression. Hence, <jats:styled-content style=\"fixed-case\">DMF<\/jats:styled-content> is a new antilymphangiogenic compound and might be used in various illnesses associated with increased lymphangiogenesis.<\/jats:p>","DOI":"10.1111\/exd.12907","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T11:06:36Z","timestamp":1450091196000},"page":"200-205","source":"Crossref","is-referenced-by-count":14,"title":["Dimethylfumarate effectively inhibits lymphangiogenesis via p21 induction and G1 cell cycle arrest"],"prefix":"10.1111","volume":"25","author":[{"given":"Eva Maria","family":"Valesky","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology Goethe\u2010University Frankfurt am Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Hrgovic","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology Goethe\u2010University Frankfurt am 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