{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:15:02Z","timestamp":1791389702235,"version":"4.3.3"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,9,29]],"date-time":"2016-09-29T00:00:00Z","timestamp":1475107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001946","name":"American Skin Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001946","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To characterize the gene expression profile of regenerated melanocytes in the narrow band UVB (NBUVB)\u2010treated vitiligo epidermis and their precursors in the hair follicle, we present here a strategy of RNA isolation from <jats:italic>in situ<\/jats:italic> melanocytes using human frozen skin. We developed a rapid immunostaining protocol using the NKI\u2010beteb antibody, which labels differentiated and precursor melanocytes, followed by fluorescent laser capture microdissection. This technique enabled the direct isolation, from melanocyte and adjacent keratinocyte populations, of satisfactory quality RNA that was successfully amplified and analysed by qRT\u2010PCR. The melanocyte\u2010specific gene transcripts <jats:italic>TYR<\/jats:italic>,<jats:italic> DCT<\/jats:italic>,<jats:italic> TYRP1<\/jats:italic> and <jats:italic>PMEL<\/jats:italic> were significantly upregulated in our NBUVB\u2010treated melanocyte samples as compared with the keratinocyte samples, while keratinocyte\u2010specific genes (<jats:italic>KRT5<\/jats:italic> and <jats:italic>KRT14<\/jats:italic>) were expressed significantly higher in the keratinocyte samples as compared with the melanocyte samples. Furthermore, in both NBUVB\u2010treated vitiligo skin and normal skin, when bulge melanocytes were compared with epidermal melanocytes, we found significantly lower expression of melanocyte\u2010specific genes and significantly higher expression of three melanocytic stem cell genes (<jats:italic>SOX9<\/jats:italic>,<jats:italic> WIF1<\/jats:italic> and <jats:italic>SFRP1),<\/jats:italic> while <jats:italic>ALCAM<\/jats:italic> and <jats:italic>ALDH1A1<\/jats:italic> transcripts did not show significant variation. We found significantly higher expression of melanocyte\u2010specific genes in the epidermis of NBUVB\u2010treated vitiligo, as compared to the normal skin. When comparing bulge melanocyte samples from untreated vitiligo, NBUVB\u2010treated vitiligo and normal skin, we did not find significant differences in the expression of melanocyte\u2010specific genes or melanocytic stem cell genes. These techniques offer valuable opportunities to study melanocytes and their precursors in vitiligo and other pigmentation disorders.<\/jats:p>","DOI":"10.1111\/exd.13072","type":"journal-article","created":{"date-parts":[[2016,5,19]],"date-time":"2016-05-19T19:16:44Z","timestamp":1463685404000},"page":"805-811","source":"Crossref","is-referenced-by-count":9,"title":["Isolating RNA from precursor and mature melanocytes from human vitiligo and normal skin using laser capture microdissection"],"prefix":"10.1111","volume":"25","author":[{"given":"Nathaniel B.","family":"Goldstein","sequence":"first","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maranke I.","family":"Koster","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"},{"name":"Charles C. Gates Center for Regenerative Medicine School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura G.","family":"Hoaglin","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"},{"name":"Charles C. Gates Center for Regenerative Medicine School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Oncology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Oncology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis R.","family":"Roop","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"},{"name":"Charles C. Gates Center for Regenerative Medicine School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Norris","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"},{"name":"Charles C. Gates Center for Regenerative Medicine School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanca A.","family":"Birlea","sequence":"additional","affiliation":[{"name":"Department of Dermatology School of Medicine University of Colorado Anschutz Medical Campus Aurora CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"792","volume-title":"Fitzpatrick's Dermatology in General Medicine","author":"Birlea SA","year":"2012"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-69361-1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12183"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.12147"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22027"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.126"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26043"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12326989"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2144\/04366ST01"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.06.064"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.4.6.697"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2011.04.008"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.233247.113"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2012.08.014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800720"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051040"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115717"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63456-6"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2011.01413.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65251-0"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2144\/01306pf01"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-298-4_2"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2013.04.023"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03233.x"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13072","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13072","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T13:01:17Z","timestamp":1695474077000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13072"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,29]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/exd.13072"],"URL":"https:\/\/doi.org\/10.1111\/exd.13072","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,29]]}}}