{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:44:42Z","timestamp":1790671482620,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,3]],"date-time":"2016-10-03T00:00:00Z","timestamp":1475452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Abstract<\/jats:title>Cathelicidin (LL<\/jats:styled-content>\u201037), Toll\u2010like receptor 2 (TLR<\/jats:styled-content>\u20102) and kallikreins (KLK<\/jats:styled-content>s) are key inflammatory mediators in rosacea. Laser or light\u2010based devices have been successfully used for rosacea. We investigated the effects of light\u2010emitting diodes (LED<\/jats:styled-content>s) on LL<\/jats:styled-content>\u201037, KLK<\/jats:styled-content>s, TLR<\/jats:styled-content>\u20102 and protease activity in cultured normal human epidermal keratinocytes (NHEK<\/jats:styled-content>s) and rosacea\u2010like mouse skin (RLMS<\/jats:styled-content>). LL<\/jats:styled-content>\u201037, KLK<\/jats:styled-content>5, KLK<\/jats:styled-content>7 and vitamin D receptor were induced by 1\u03b1, 25\u2010dihydroxyvitamin D3<\/jats:sub> (VD<\/jats:styled-content>3<\/jats:sub>) and TLR<\/jats:styled-content>\u20102 by Ad\u2010CMV<\/jats:styled-content> transfection in cultured NHEK<\/jats:styled-content>s. NHEK<\/jats:styled-content>s were subjected to LED<\/jats:styled-content> irradiation at differing wavelengths (480\u2013940\u00a0nm) and fluences (1\u201340\u00a0J\/cm2<\/jats:sup>). Inflammatory mediators were analysed with RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> and real\u2010time PCR<\/jats:styled-content> and protease activity analysis and immunocytofluorescence staining were performed for NHEK<\/jats:styled-content>s. Changes in RLMS<\/jats:styled-content> induced by LL<\/jats:styled-content>\u201037 peptide were evaluated with real\u2010time PCR<\/jats:styled-content>, immunohistochemical staining and enzyme\u2010linked immunosorbent assay. In NHEK<\/jats:styled-content>s, LED<\/jats:styled-content> at 630 and 940\u00a0nm significantly attenuated LL<\/jats:styled-content>37, KLK<\/jats:styled-content>5 and TLR<\/jats:styled-content>\u20102 mRNA<\/jats:styled-content> expressions. Protease activity was significantly suppressed at 630, 850 and 940\u00a0nm. In the RLMS<\/jats:styled-content>, LL<\/jats:styled-content>\u201037, KLK<\/jats:styled-content>5 and PAR<\/jats:styled-content>\u20102 mRNA<\/jats:styled-content> expressions significantly decreased at 24 and 48\u00a0hours after LED<\/jats:styled-content> irradiation was performed three times at 630 and 940\u00a0nm. mCAMP<\/jats:styled-content> and IL<\/jats:styled-content>\u20108 protein levels and protease activity after LED<\/jats:styled-content> irradiation were lower than those in RLMS<\/jats:styled-content> control groups. LED<\/jats:styled-content> at 630 and 940\u00a0nm downregulated TLR<\/jats:styled-content>\u20102, KLK<\/jats:styled-content>5 and LL<\/jats:styled-content>\u201037 expressions and protease activity in NHEK<\/jats:styled-content> and RLMS<\/jats:styled-content>. Thus, LED<\/jats:styled-content>s may be promising for rosacea treatment. However, clinical trials are required for further study.<\/jats:p>","DOI":"10.1111\/exd.13133","type":"journal-article","created":{"date-parts":[[2016,6,17]],"date-time":"2016-06-17T14:37:01Z","timestamp":1466174221000},"page":"956-961","source":"Crossref","is-referenced-by-count":27,"title":["Light\u2010emitting diodes downregulate cathelicidin, kallikrein and toll\u2010like receptor 2 expressions in keratinocytes and rosacea\u2010like mouse skin"],"prefix":"10.1111","volume":"25","author":[{"given":"Jee\u2010Bum","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Dermatology Chonnam National University Medical School Gwangju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Hyeon","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chonnam National University Medical School Gwangju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki 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