{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T22:37:36Z","timestamp":1774910256020,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T00:00:00Z","timestamp":1480377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["NSC 101\u20102320\u2010B\u2010030\u2010005\u2010MY3"],"award-info":[{"award-number":["NSC 101\u20102320\u2010B\u2010030\u2010005\u2010MY3"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 103\u20102321\u2010B\u2010195\u2010001\u2010"],"award-info":[{"award-number":["MOST 103\u20102321\u2010B\u2010195\u2010001\u2010"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004861","name":"Mackay Memorial Hospital","doi-asserted-by":"publisher","award":["MMH\u2010104\u201030"],"award-info":[{"award-number":["MMH\u2010104\u201030"]}],"id":[{"id":"10.13039\/501100004861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004861","name":"Mackay Memorial Hospital","doi-asserted-by":"publisher","award":["MMH\u2010103\u201002"],"award-info":[{"award-number":["MMH\u2010103\u201002"]}],"id":[{"id":"10.13039\/501100004861","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"Abstract<\/jats:title>Solar ultraviolet (UV<\/jats:styled-content>) light has been recognized as the important environmental hazard and contributes to diverse skin damage such as cell death, photoageing and even carcinogenesis. Revelation of harmful responses attributed to UVA<\/jats:styled-content> radiation has promoted the development of photoprotective agents against UVA<\/jats:styled-content>\u2010induced skin damage. In the present study, we tried to evaluate the potential protective effects of a synthetic green fluorescent protein (GFP<\/jats:styled-content>) chromophore derivative, 4\u2010chlorobenzyldene\u20101, 2\u2010dimethylimidazolinone (Cl\u2010BDI<\/jats:styled-content>, called TC<\/jats:styled-content>\u201022) on UVA<\/jats:styled-content>\u2010 and UVB<\/jats:styled-content>\u2010 induced stress responses in skin. The HaCaT keratinocytes were used to evaluate the cellular effects. Zebrafish (Danio rerio<\/jats:italic>), which is regarded as a useful and cost\u2010effective alternative to some mammalian models, was applied as the in vivo animal model. In HaCaT keratinocytes, TC<\/jats:styled-content>\u201022 was able to obviously decrease UVA<\/jats:styled-content>\u2010induced cell death. Dissection of the UVA<\/jats:styled-content>\u2010induced signalling pathways revealed that TC<\/jats:styled-content>\u201022 could suppress the activation of JNK<\/jats:styled-content> and caspase 3, but not of ERK<\/jats:styled-content> and p38. Reduction of UVA<\/jats:styled-content>\u2010induced cleavage of caspase 3 and sub\u2010G1 phase accumulation by pretreatment of TC<\/jats:styled-content>\u201022 was also observed. In zebrafish, we showed that UVA<\/jats:styled-content> irradiation could decrease the survival and hatching rate, suppress heart beats of embryos and enhance the pigmentation of larvae. Pretreatment of TC<\/jats:styled-content>\u201022 could significantly reverse UVA<\/jats:styled-content>\u2010induced the suppression in hatching of eggs and heart beating of embryos and also lowered the UVA<\/jats:styled-content>\u2010induced pigmentation in zebrafish. Collectively, we demonstrate that TC<\/jats:styled-content>\u201022, a GFP<\/jats:styled-content> chromophore derivative, can ameliorate the UVA<\/jats:styled-content>\u2010induced stress responses in both epidermal keratinocytes and zebrafish, suggesting the potential use of TC<\/jats:styled-content>\u201022 in photoprotection in the future.<\/jats:p>","DOI":"10.1111\/exd.13168","type":"journal-article","created":{"date-parts":[[2016,8,11]],"date-time":"2016-08-11T14:18:46Z","timestamp":1470925126000},"page":"983-990","source":"Crossref","is-referenced-by-count":6,"title":["Green fluorescent protein chromophore derivative suppresses ultraviolet A\u2010induced JNK<\/scp>\u2010signalling and apoptosis in keratinocytes and adverse effects in zebrafish embryos"],"prefix":"10.1111","volume":"25","author":[{"given":"Nan\u2010Lin","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Medicine Mackay Medical College New Taipei City Taiwan"},{"name":"Department of Dermatology Mackay Memorial Hospital Taipei Taiwan"},{"name":"Mackay Junior College of Medicine, Nursing and Management New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Te\u2010An","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology Hsinchu Mackay Memorial Hospital Hsinchu Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng\u2010Fen","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Basic Medicine Fu Jen Catholic University New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Ju","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry Fu Jen Catholic University New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Fragrance and Cosmetic Science Kaohsiung Medical University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tun\u2010Cheng","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Taiwan Normal University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chieh\u2010Chen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shin Kong Wu Ho\u2010Su Memorial Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Feng","family":"Hung","sequence":"additional","affiliation":[{"name":"School of Medicine Fu Jen Catholic University New Taipei City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-009-0994-y"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2004.07.019"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12388"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.60.8.0004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(99)00077-8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf200931t"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(94)90091-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1606"},{"key":"e_1_2_9_10_1","first-page":"e21","volume":"134","author":"Li Q","year":"2014","journal-title":"J Invest 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