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Omega\u20103 polyunsaturated fatty acids show potential to reduce UVR<\/jats:styled-content> suppression of CMI<\/jats:styled-content> in mice and humans, potentially through modulation of LC<\/jats:styled-content> migration. Our objectives were to examine whether eicosapentaenoic acid (EPA<\/jats:styled-content>) ingestion influences UV<\/jats:styled-content>\u2010mediated effects on epidermal LC<\/jats:styled-content> numbers and levels of immunomodulatory mediators including prostaglandin (PG<\/jats:styled-content>)D2<\/jats:sub>, which is expressed by LC<\/jats:styled-content>. In a double\u2010blind randomised controlled study, healthy individuals took 5\u2010g EPA<\/jats:styled-content>\u2010rich (n=40) or control (n=33) lipid for 12\u00a0weeks; UVR<\/jats:styled-content>\u2010exposed and unexposed skin samples were taken pre\u2010 and postsupplementation. Epidermal LC<\/jats:styled-content> numbers were assessed by immunofluorescence for CD<\/jats:styled-content>1a, and skin blister fluid PG<\/jats:styled-content> and cytokines were quantified by LC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> and Luminex assay, respectively. Presupplementation, UVR<\/jats:styled-content> reduced mean (SEM<\/jats:styled-content>) LC<\/jats:styled-content> number\/mm2<\/jats:sup> from 913 (28) to 322 (40) (P<\/jats:italic><.001), and mean PGD<\/jats:styled-content>2<\/jats:sub> level by 37% from 8.1 (11.6) to 5.1 (5.6)\u00a0pg\/\u03bcL; P<\/jats:italic><.001), while IL<\/jats:styled-content>\u20108 level increased (P<\/jats:italic><.001). Despite confirmation of EPA<\/jats:styled-content> bioavailability in red blood cells and skin in the active group, no between\u2010group effect of EPA<\/jats:styled-content> was found on UVR<\/jats:styled-content> modulation of LC<\/jats:styled-content> numbers, PGD<\/jats:styled-content>2<\/jats:sub> or cytokine levels postsupplementation. Thus, no evidence was found for EPA<\/jats:styled-content> reduction of photoimmunosuppression through an impact on epidermal LC<\/jats:styled-content> numbers. Intriguingly, UVR<\/jats:styled-content> exposure substantially reduced cutaneous PGD<\/jats:styled-content>2<\/jats:sub> levels in humans, starkly contrasting with reported effects of UVR<\/jats:styled-content> on other skin PG<\/jats:styled-content>. Lowered PGD<\/jats:styled-content>2<\/jats:sub> levels could reflect LC<\/jats:styled-content> loss from the epidermis and\/or altered dendritic cell activity and may be relevant for phototherapy of skin disease.<\/jats:p>","DOI":"10.1111\/exd.13177","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T11:22:56Z","timestamp":1472556176000},"page":"962-968","source":"Crossref","is-referenced-by-count":14,"title":["Effect of oral eicosapentaenoic acid on epidermal Langerhans cell numbers and PGD<\/scp>2<\/sub> production in UVR<\/scp>\u2010exposed human skin: a randomised controlled study"],"prefix":"10.1111","volume":"25","author":[{"given":"Suzanne M.","family":"Pilkington","sequence":"first","affiliation":[{"name":"Centre for Dermatology Institute of Inflammation and Repair University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil 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