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Featured cutaneous transduction\/transfection methods include viral modalities (ie adenoviruses, adeno\u2010associated viruses, retroviruses and lentiviruses) and conventional non\u2010viral modalities (ie naked <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> injections, microseeding, liposomal reagents, particle bombardment and electroporation). Also explored are emerging technologies, focusing on the exciting capabilities of wound diagnostics such as pyrosequencing as well as site\u2010specific nuclease editing tools such as <jats:styled-content style=\"fixed-case\">CRISPR<\/jats:styled-content>\u2010Cas9 used to both transiently and permanently genetically modify resident wound bed cells. Additionally, new non\u2010viral transfection methods (ie conjugated nanoparticles, multi\u2010electrode arrays, and microfabricated needles and nanowires) are discussed that can potentially facilitate more efficient and safe transgene delivery to skin but also represent significant advances broadly to tissue regeneration research.<\/jats:p>","DOI":"10.1111\/exd.13185","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T13:52:23Z","timestamp":1472565143000},"page":"179-185","source":"Crossref","is-referenced-by-count":22,"title":["A review of genetic engineering biotechnologies for enhanced chronic wound healing"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4686-611X","authenticated-orcid":false,"given":"John W.","family":"Sessions","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Brigham Young University  Provo UT 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