{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:16:04Z","timestamp":1790745364406,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prevalence of food allergies worldwide has increased recently. Epicutaneous sensitization to antigen could be a method to study food allergy. To clarify the mechanisms of food allergy, we established a mouse model of epicutaneous sensitization using ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were sensitized by three\u2010time application of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> to tape\u2010stripped skin (1\u2010week sensitization at 2\u2010week intervals) and oral challenge of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> undertaken. Rectal temperature was monitored. Blood and tissue (skin and jejunum) of challenged mice were taken. Numbers of mast cells (<jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s) and basophils were counted. Serum and\/or tissue levels of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> \u2010specific IgE and IgG antibodies and several cytokines were measured using enzyme\u2010linked immunoassay kits. <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> and basophil depletion experiments were undertaken. In <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\/epicutaneous\u2010sensitized and orally challenged mice, systemic anaphylaxis (as evidenced by reduced rectal temperature) was observed. Levels of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010specific IgE and IgG antibodies were increased in these mice, as were increased number of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s and basophils. Serum levels of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> protease 1 were increased significantly. Basophil and <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> depletion experiments revealed that they both participate in reactions. Increased production of thymic stromal lymphopoietin (<jats:styled-content style=\"fixed-case\">TSLP<\/jats:styled-content>) at skin sites of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> sensitization was noted. We speculate that <jats:styled-content style=\"fixed-case\">TSLP<\/jats:styled-content> produced from epidermal cells during antigen sensitization can enable basophils to promote a T helper (Th)2 immune reaction, leading to and systemic anaphylaxis by antigen\u2010specific IgE\u2010bearing <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s. This <jats:styled-content style=\"fixed-case\">TSLP<\/jats:styled-content>\u2010basophils\u2010<jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content> axis could be a novel therapeutic target against food allergy.<\/jats:p>","DOI":"10.1111\/exd.13279","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T15:22:56Z","timestamp":1482160976000},"page":"778-784","source":"Crossref","is-referenced-by-count":14,"title":["Basophils and mast cells are crucial for reactions due to epicutaneous sensitization to ovalbumin"],"prefix":"10.1111","volume":"26","author":[{"given":"Rie","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Dermatology Tokyo Medical and Dental University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Igawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical and Dental University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaro","family":"Handa","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical and Dental University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takichi","family":"Munetsugu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical and Dental University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Satoh","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Yokozeki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Tokyo Medical and Dental University Graduate School of Medicine  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.05.026"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02304.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.039"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12477"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1001\/jama.1997.03550220094013","volume":"278","author":"Sampson H. 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