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The four mA<\/jats:styled-content>bs, tentatively named as COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs, showed speckled subepidermal staining in addition to linear BMZ<\/jats:styled-content> staining of normal human skin and bovine cornea, a characteristic immunofluorescence feature of COL<\/jats:styled-content>7, but showed no reactivity with COL<\/jats:styled-content>7 by in vitro biochemical analyses. Taking advantage of the phenomenon that COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs did not react with mouse BMZ<\/jats:styled-content>, we compared immunofluorescence reactivity between wild\u2010type and COL<\/jats:styled-content>7\u2010rescued humanized mice and found that COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs reacted with BMZ<\/jats:styled-content> of COL<\/jats:styled-content>7\u2010rescued humanized mice. In ELISA<\/jats:styled-content>s, COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs reacted with intact triple\u2010helical mammalian recombinant protein (RP) of COL<\/jats:styled-content>7 but not with bacterial RP. Furthermore, COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs did not react with COL<\/jats:styled-content>7 within either cultured DJM<\/jats:styled-content>\u20101 cells or basal cells of skin of a bullous dermolysis of the newborn patient. These results confirmed that COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs reacted with human and bovine COL<\/jats:styled-content>7. The epitopes for COL<\/jats:styled-content>7\u2010like mA<\/jats:styled-content>bs were considered to be present only on mature COL<\/jats:styled-content>7 after secretion from keratinocytes and deposition to BMZ<\/jats:styled-content> and to be easily destroyed during immunoblotting procedure. Additional studies indicated association of the speckled subepidermal staining with both type IV<\/jats:styled-content> collagen and elastin. These unique anti\u2010COL<\/jats:styled-content>7 mA<\/jats:styled-content>bs should be useful in studies of both normal and diseased conditions, particularly dystrophic epidermolysis bullosa, which produces only immature COL<\/jats:styled-content>7.<\/jats:p>","DOI":"10.1111\/exd.13306","type":"journal-article","created":{"date-parts":[[2017,1,23]],"date-time":"2017-01-23T07:46:42Z","timestamp":1485157602000},"page":"811-819","source":"Crossref","is-referenced-by-count":2,"title":["Unique mouse monoclonal antibodies reactive with maturation\u2010related epitopes on type VII<\/scp> collagen"],"prefix":"10.1111","volume":"26","author":[{"given":"Taihei","family":"Hayakawa","sequence":"first","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"},{"name":"Department of Oral and Maxillofacial Surgery School of Dentistry Aichi\u2010Gakuin University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Hirako","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwesi","family":"Teye","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsunari","family":"Tsuchisaka","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norito","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Karashima","sequence":"additional","affiliation":[{"name":"Department of Dermatology Kurume University School of Medicine Kurume University Institute of Cutaneous Cell Biology Kurume Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minori","family":"Kaneda","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Oyu","sequence":"additional","affiliation":[{"name":"Division of Biological Science Graduate School of Science Nagoya University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiharu","family":"Tateishi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Department of Dermatology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayano","family":"Yonamine","sequence":"additional","affiliation":[{"name":"Department of Dermatology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Shinkuma","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hokkaido University Graduate School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hokkaido University Graduate School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Fukano","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery School of Dentistry Aichi\u2010Gakuin University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Shimozato","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery School of Dentistry Aichi\u2010Gakuin University Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngon T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Stanford University School of Medicine Center for Clinical Sciences Research Stanford CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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