{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:45:07Z","timestamp":1772160307608,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,9]],"date-time":"2017-07-09T00:00:00Z","timestamp":1499558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008271","name":"LEO Pharma Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Abstract<\/jats:title>\n \n The gene expression time\u2010course of repeated challenge of contact allergy (\n CA<\/jats:styled-content>\n ) remains largely unknown. Therefore, using diphenylcyclopropenone (\n DPCP<\/jats:styled-content>\n ) as model allergen in healthy humans we set out to examine: (i) the monotonous and complex gene expression time\u2010course trajectories following repeated DPCP challenges to find the predominant gene expression pattern, (ii) the time\u2010course of cell infiltration following repeated\n DPCP<\/jats:styled-content>\n challenges and (iii) the transcriptome of a repeated\n CA<\/jats:styled-content>\n exposure model. We obtained punch biopsies from control and\n DPCP<\/jats:styled-content>\n \u2010exposed skin from ten\n DPCP<\/jats:styled-content>\n sensitized individuals at 5\u20106 monthly elicitation challenges. Biopsies were used for microarray gene expression profiling, histopathology and immunohistochemical staining. Validation of microarray data by\n qRT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n was performed on 15 selected genes. Early gene expression time points were also validated in an independent data set. An increasing and decreasing trend in gene expression followed by a plateau was predominantly observed during repeated\n DPCP<\/jats:styled-content>\n challenges. Immune responses reached a plateau after two challenges histopathologically, immunohistochemically and in the time\u2010course gene expression analysis. Transcriptional responses over time revealed a Th1\/Th17 polarization as three upstream regulators (\n IFN<\/jats:styled-content>\n \u2010\u03b3,\n IL<\/jats:styled-content>\n \u20101 and\n IL<\/jats:styled-content>\n \u201017) activated most of the top upregulated genes. Of the latter genes, 9 of 10 were the same throughout the time course. Excellent correlations between array and\n PCR<\/jats:styled-content>\n data were observed. The transcriptional responses to\n DPCP<\/jats:styled-content>\n over time followed a monotonous pattern. This response pattern confirms and supports the newly reported clinical time\u2010course observations in de novo\u2010sensitized individuals showing a plateau response, and thus, there is concordance between clinical response, histopathology, immunohistochemistry and microarray gene expression in volunteers de novo\u2010sensitized to\n DPCP<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/exd.13345","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T03:26:58Z","timestamp":1490930818000},"page":"926-933","source":"Crossref","is-referenced-by-count":6,"title":["The gene expression and immunohistochemical time\u2010course of diphenylcyclopropenone\u2010induced contact allergy in healthy humans following repeated epicutaneous challenges"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3689-7350","authenticated-orcid":false,"given":"Kristian F.","family":"Mose","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital University of Southern Denmark Odense C Denmark"},{"name":"Dermatological Investigations Scandinavia Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Burton","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads","family":"Thomassen","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flemming","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital University of Southern Denmark Odense C Denmark"},{"name":"Dermatological Investigations Scandinavia Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torben A.","family":"Kruse","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qihua","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics Odense University Hospital University of Southern Denmark Odense C Denmark"},{"name":"Epidemiology, Biostatistics and Biodemography Department of Public Health University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lone","family":"Skov","sequence":"additional","affiliation":[{"name":"Department of Dermato\u2010Allergology Herlev and Gentofte Hospital University of Copenhagen Hellerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mads A.","family":"R\u00f8pke","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pharmacology and Molecular Biomedicine LEO Pharma A\/S Ballerup Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Litman","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pharmacology and Molecular Biomedicine LEO Pharma A\/S Ballerup Denmark"},{"name":"Department of Immunology and Microbiology University of Copenhagen Copenhagen N Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ole","family":"Clemmensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjarne W.","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology Odense University Hospital University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"Friedmann","sequence":"additional","affiliation":[{"name":"Division of Infection, Inflammation & Immunity Sir Henry Wellcome Laboratories Southampton University Hospitals NHS Trust Southampton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus E.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy Centre Odense University Hospital University of Southern Denmark Odense C Denmark"},{"name":"Dermatological Investigations Scandinavia Odense University Hospital University of Southern Denmark Odense C Denmark"},{"name":"Centre for Innovative Medical Technology Institute of Clinical Research University of Southern Denmark Odense C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/261514"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700902"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.196"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1986.tb01206.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.18.8497"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2011.02.014"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14949"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1997.tb00312.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2343"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.08.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-387827-4.00004-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.6581.1"}],"container-title":["Experimental 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