{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:02:02Z","timestamp":1771639322179,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inhibition of transforming growth factor (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>)\u2010\u03b21 signalling may be one of the most reliable approaches to treat skin fibrosis of scleroderma. Although there have been many basic researches of <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 blockade reagents, few of them were proved to have inhibitory effects on fibrosis both in vitro and in vivo. In this study, we randomly chose four commercially available low molecular weight\u00a0compounds\u00a0(Repsox, <jats:styled-content style=\"fixed-case\">LY<\/jats:styled-content>2109761, <jats:styled-content style=\"fixed-case\">LY<\/jats:styled-content>364947 and K02288) from <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21 inhibitor library, and compared their antifibrotic effects in vitro and in vivo. We demonstrated that Repsox has the most potent inhibitory effects on <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2\u2010induced expression of <jats:styled-content style=\"fixed-case\">CTGF<\/jats:styled-content> and collagen of cultured normal dermal fibroblasts in vitro and their constitutive overexpression of scleroderma fibroblast in vitro. In addition, Repsox could attenuate skin fibrosis by bleomycin in vivo, via the downregulation of <jats:styled-content style=\"fixed-case\">CTGF<\/jats:styled-content> or collagen. Our results may facilitate clinical trial of Repsox against fibrotic diseases in future.<\/jats:p>","DOI":"10.1111\/exd.13366","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T13:42:10Z","timestamp":1492522930000},"page":"1139-1143","source":"Crossref","is-referenced-by-count":19,"title":["Transforming growth factor \u03b2\u2010inhibitor Repsox downregulates collagen expression of scleroderma dermal fibroblasts and prevents bleomycin\u2010induced mice skin fibrosis"],"prefix":"10.1111","volume":"26","author":[{"given":"Maho","family":"Ide","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9230-6207","authenticated-orcid":false,"given":"Masatoshi","family":"Jinnin","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Tomizawa","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikko","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinobu","family":"Hashizume","sequence":"additional","affiliation":[{"name":"RIKEN Program for Drug Discovery and Medical Technology Platforms  Wako Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihide","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Tokyo Graduate School of Medicine  Bunkyo\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Ihn","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,25]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-198901040-00011"},{"key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0889-857X(21)01042-5","volume":"16","author":"Mauch C.","year":"1990","journal-title":"Rheum. 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