{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:12:37Z","timestamp":1790161957915,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In human dendritic cells (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s), we previously demonstrated in vitro that syndecan\u20101 (<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1) is downregulated during maturation correlating with enhanced motility.<\/jats:p><jats:p>We investigated the effects of<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 on<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>migration in vivo during<jats:styled-content style=\"fixed-case\">TNCB<\/jats:styled-content>(2,4,6\u2010trinitro\u20101\u2010chlorobenzene)\u2010induced cutaneous hypersensitivity reaction (<jats:styled-content style=\"fixed-case\">CHS<\/jats:styled-content>) in mice.<\/jats:p><jats:p>We show that<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>in<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2010deficient mice migrated faster and at a higher rate to lymph nodes draining the hapten\u2010painted skin. Adoptive transfer of<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2010deficient hapten\u2010 and fluorochrome\u2010labelled<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>into wild\u2010type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) mice led to increased and faster migration of<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>to paracortical lymph nodes, and to a stronger<jats:styled-content style=\"fixed-case\">CHS<\/jats:styled-content>compared to<jats:styled-content style=\"fixed-case\">WT DC<\/jats:styled-content>. In<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2212\/\u2212 mice,<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>7 remains longer on the<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>surface within the first 15\u2010minutes maturation (after<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced maturation). In addition, a time\u2010dependent upregulation of<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>2,<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3,<jats:styled-content style=\"fixed-case\">VCAM<\/jats:styled-content>1 and talin was found during maturation in<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2212\/\u2212<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>. However, no difference in T\u2010cell\u2010stimulating capacity of<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2010deficient<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>was found compared to<jats:styled-content style=\"fixed-case\">WT DC<\/jats:styled-content>. Mechanistically,<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2010deficient<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>showed enhanced migration towards<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>21 and<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>19. This may result from functional overexpression of<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>7 in<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2212\/\u2212<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>. Increased and accelerated migration of otherwise functionally intact<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1\u2010deficient<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>leads to an exacerbated<jats:styled-content style=\"fixed-case\">CHS<\/jats:styled-content>. Based on our results, we conclude that<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 on<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>negatively regulates<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>migration.<\/jats:p>","DOI":"10.1111\/exd.13374","type":"journal-article","created":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T16:58:15Z","timestamp":1493398695000},"page":"1060-1067","source":"Crossref","is-referenced-by-count":18,"title":["Syndecan\u20101 regulates dendritic cell migration in cutaneous hypersensitivity to haptens"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0246-7646","authenticated-orcid":false,"given":"Marco","family":"Averbeck","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venerology and Allergology Universit\u00e4tsklinikum Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Department of Environmental Immunology UFZ \u2010 Helmholtz Centre for Environmental Research Leipzig\u2010Halle Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"B\u00fchligen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venerology and Allergology Universit\u00e4tsklinikum Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"G\u00f6tte","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics M\u00fcnster University Hospital M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan C.","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venerology and Allergology Universit\u00e4tsklinikum Leipzig Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Polte","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venerology and Allergology Universit\u00e4tsklinikum Leipzig Leipzig Germany"},{"name":"Department of Environmental Immunology UFZ \u2010 Helmholtz Centre for Environmental Research Leipzig\u2010Halle Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/32588"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104538"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.22.012703.104558"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.223"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.04.004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.04.017"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.06.014"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(10)78009-5"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161747"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi971125s"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S1016-8478(23)07324-7","volume":"24","author":"Bartlett A. 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