{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:52:39Z","timestamp":1778705559750,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T00:00:00Z","timestamp":1502755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pruritus is a major symptom of several dermatological diseases but has limited therapeutic options available. Animal models replicating the pathophysiology of pruritus are needed to support the development of new drugs. Induction of pruritus by chloroquine (<jats:styled-content style=\"fixed-case\">CQ<\/jats:styled-content>) in mice is widely used, although, as with similar models, it has low throughput and does not distinguish between antipruritic effects and confounding factors such as sedation. To overcome these issues, we incorporated into the model an automated system that measures both scratching and locomotor behaviour simultaneously. We combined this system with the determination of <jats:styled-content style=\"fixed-case\">CQ<\/jats:styled-content> levels in different tissues to understand the impact of the route of <jats:styled-content style=\"fixed-case\">CQ<\/jats:styled-content> administration on the pruritogenic response. We concluded that whereas oral <jats:styled-content style=\"fixed-case\">CQ<\/jats:styled-content> does not induce pruritus due to insufficient skin levels, the bell\u2010shaped curve of pruritus observed following subcutaneous administration is due to toxicity at high doses. We validated the model with several drugs currently used in humans: nalfurafine, aprepitant, cyproheptadine and amitriptyline. By comparing the effects of the drugs on both scratching and locomotor activity, we concluded that nalfurafine and aprepitant can exhibit efficacy at doses devoid of central effects, whereas central effects drove the efficacy of the other two drugs. This was further confirmed using non\u2010brain\u2010penetrant drugs. Moreover, as anticipated, anti\u2010inflammatory drugs showed no efficacy. In conclusion, the use of an automated integrated behavioural assessment in <jats:styled-content style=\"fixed-case\">CQ<\/jats:styled-content>\u2010induced pruritus makes the assay suitable for screening purposes and allows for a correct interpretation of the antipruritic effect of the compounds evaluated.<\/jats:p>","DOI":"10.1111\/exd.13392","type":"journal-article","created":{"date-parts":[[2017,6,12]],"date-time":"2017-06-12T09:52:45Z","timestamp":1497261165000},"page":"1105-1111","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of the chloroquine\u2010induced mouse model of pruritus using an automated behavioural system"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3595-0327","authenticated-orcid":false,"given":"Gema","family":"Tarras\u00f3n","sequence":"first","affiliation":[{"name":"Skin Biology and Pharmacology Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Carcasona","sequence":"additional","affiliation":[{"name":"Skin Biology and Pharmacology Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Eichhorn","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bibiana","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amadeu","family":"Gavald\u00e0","sequence":"additional","affiliation":[{"name":"Skin Biology and Pharmacology Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3546-4924","authenticated-orcid":false,"given":"N\u00faria","family":"Godessart","sequence":"additional","affiliation":[{"name":"Skin Biology and Pharmacology Almirall R&amp;D Center  Sant Feliu de Llobregat Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1950"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28553"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0305"},{"key":"e_1_2_8_5_1","first-page":"164","volume":"85","author":"Lavery M. 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