{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:41:18Z","timestamp":1789904478599,"version":"4.0.1"},"reference-count":12,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["#31003A_135689"],"award-info":[{"award-number":["#31003A_135689"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The potentially severe side effects of systemic corticosteroids and immunosuppressants used in Pemphigus vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) call for novel therapeutic approaches. In this context, pharmacological inhibition of major pathogenic signalling effectors represents a promising alternative. However, we have also shown that overinhibition of effectors required for epidermal homeostasis can exacerbate <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> pathophysiology implicating transepidermal keratinocyte fragility. A feedforward target validation therefore preferentially includes studies on knockout mouse models. We previously reported on successful amelioration of <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> blisters following inhibition of non\u2010apoptotic, low\u2010level caspase\u20103. Here, we use conditional, keratinocyte\u2010specific caspase\u20103\u2010deficient mice (casp3<jats:styled-content style=\"fixed-case\"><jats:sup>EKO<\/jats:sup><\/jats:styled-content>) to demonstrate (i) absence of keratinocyte fragility upon injection of the potent Dsg3\u2010specific antibody <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content>23 and (ii) amelioration of blistering on the background of known signalling effectors. Our results provide the experimental proof of concept justifying translation of the caspase\u20103 inhibitor approach into <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> clinical trials.<\/jats:p>","DOI":"10.1111\/exd.13458","type":"journal-article","created":{"date-parts":[[2017,11,3]],"date-time":"2017-11-03T15:54:01Z","timestamp":1509724441000},"page":"1274-1277","source":"Crossref","is-referenced-by-count":12,"title":["Feasibility study for clinical application of caspase\u20103 inhibitors in Pemphigus vulgaris"],"prefix":"10.1111","volume":"26","author":[{"given":"William V.J.","family":"Hariton","sequence":"first","affiliation":[{"name":"Department of BioMedical Research Molecular Dermatology and Stem Cell Research University of Bern  Bern Switzerland"},{"name":"Department of Dermatology, Inselspital Bern University Hospital  Bern Switzerland"},{"name":"DermFocus, Vetsuisse Faculty University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Galichet","sequence":"additional","affiliation":[{"name":"Department of BioMedical Research Molecular Dermatology and Stem Cell Research University of Bern  Bern Switzerland"},{"name":"Department of Dermatology, Inselspital Bern University Hospital  Bern Switzerland"},{"name":"DermFocus, Vetsuisse Faculty University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Vanden Berghe","sequence":"additional","affiliation":[{"name":"Molecular Signaling and Cell Death Unit VIB Center for Inflammation Research  Ghent Belgium"},{"name":"Department of Biomedical Molecular Biology Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Overmiller","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Cutaneous Biology Thomas Jefferson University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My G.","family":"Mahoney","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Cutaneous Biology Thomas Jefferson University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Declercq","sequence":"additional","affiliation":[{"name":"Molecular Signaling and Cell Death Unit VIB Center for Inflammation Research  Ghent Belgium"},{"name":"Department of Biomedical Molecular Biology Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1606-3359","authenticated-orcid":false,"given":"Eliane J.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of BioMedical Research Molecular Dermatology and Stem Cell Research University of Bern  Bern Switzerland"},{"name":"Department of Dermatology, Inselspital Bern University Hospital  Bern Switzerland"},{"name":"DermFocus, Vetsuisse Faculty University of Bern  Bern Switzerland"},{"name":"Institute of Animal Pathology Vetsuisse Faculty University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,27]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-015-0541-1"},{"key":"e_1_2_2_3_1","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1242\/jcs.111.17.2529","volume":"111","author":"Koch P.J.","year":"1998","journal-title":"J. Cell Sci."},{"key":"e_1_2_2_4_1","author":"Spindler V.","year":"2017","journal-title":"J. Invest. Dermatol."},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602973103"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12290"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601224"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.224"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2017.19"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119809"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.299"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00632.x"},{"key":"e_1_2_2_13_1","first-page":"473","volume":"66","author":"Kudelova J.","year":"2015","journal-title":"J. Physiol. Pharmacol."}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13458","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T12:41:15Z","timestamp":1694608875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":12,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/exd.13458"],"URL":"https:\/\/doi.org\/10.1111\/exd.13458","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]}}}