{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T10:42:09Z","timestamp":1776422529757,"version":"3.51.2"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003072","name":"LOEWE Zentrum AdRIA","doi-asserted-by":"publisher","award":["245\/10\u201021"],"award-info":[{"award-number":["245\/10\u201021"]}],"id":[{"id":"10.13039\/501100003072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FOR 2497"],"award-info":[{"award-number":["FOR 2497"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pemphigus vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) is a severe autoimmune blistering disease of the skin and mucous membranes. As autoantibodies play an essential role in the disease pathogenesis, the serological detection of anti\u2010desmoglein 3 IgG represents a central tool in the diagnosis of the disease. In this study, we show the validation of a novel lateral flow immunoassay (<jats:styled-content style=\"fixed-case\">LFIA<\/jats:styled-content>) which rapidly detects anti\u2010desmoglein 3 (Dsg3) IgG in human serum. In contrast to other diagnostic procedures, the assay is compact and simple to perform and delivers a fast\u00a0\u201dyes\u201d or \u201cno\u201d answer within 10\u00a0minutes without additional hardware requirements for test evaluation. For validation, a blinded collection of 200 sera including 100 sera from 14 <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content> patients, 75 sera from 24 bullous pemphigoid patients and 25 sera from 6 patients with pemphigus foliaceus collected at different time points during disease was used. Presence or non\u2010presence of anti\u2010Dsg3 IgG within sera was confirmed using a commercially available Dsg3\u2010<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. For qualitative evaluation, Dsg3\u2010<jats:styled-content style=\"fixed-case\">LFIA<\/jats:styled-content> test results were assessed by two independent groups of human observers. Furthermore, quantitative evaluation using <jats:styled-content style=\"fixed-case\">POCS<\/jats:styled-content>can reader was applied. The Dsg3\u2010<jats:styled-content style=\"fixed-case\">LFIA<\/jats:styled-content> demonstrated reliable test results with a sensitivity and specificity of 78.1% and 97.1%, respectively. Test results from <jats:styled-content style=\"fixed-case\">POCS<\/jats:styled-content>can and human observers showed a substantial agreement. The Dsg3\u2010<jats:styled-content style=\"fixed-case\">LFIA<\/jats:styled-content> represents a new diagnostic tool for the immediate and reliable detection of anti\u2010desmoglein 3 serum IgG autoantibodies that does not require additional hardware. Further prospective trials are warranted to validate the Dsg3 <jats:styled-content style=\"fixed-case\">LFIA<\/jats:styled-content> in pemphigus.<\/jats:p>","DOI":"10.1111\/exd.13488","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T14:56:42Z","timestamp":1514300202000},"page":"233-237","source":"Crossref","is-referenced-by-count":8,"title":["A novel lateral flow immunoassay for the rapid detection of anti\u2010Dsg3 IgG serum autoantibodies in pemphigus vulgaris"],"prefix":"10.1111","volume":"27","author":[{"given":"Thomas","family":"Schmidt","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University  Marburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Mauracher","sequence":"additional","affiliation":[{"name":"Milenia Biotec  Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lena","family":"Bender","sequence":"additional","affiliation":[{"name":"Milenia Biotec  Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"Greene","sequence":"additional","affiliation":[{"name":"Institute of Biometry and Statistics Philipps\u2010University Marburg  Marburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Kurzhals","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University  Marburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00fcdiger","family":"Eming","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University  Marburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Dostatni","sequence":"additional","affiliation":[{"name":"Milenia Biotec  Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hertl","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Philipps\u2010University  Marburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2]]},"reference":[{"key":"e_1_2_8_2_1","author":"Pollmann R.","year":"2018","journal-title":"Clin. 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