{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:47:56Z","timestamp":1783648076186,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["DK MOLIN\u2010W1241"],"award-info":[{"award-number":["DK MOLIN\u2010W1241"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vivo tumor models are essential for studying the biology of cancer, identifying tumor targets and evaluating antitumor drugs. Considering the request for the minimisation of animal experiments and following the \u201c3R\u201d\u2010rule (\u201creplacement,\u201d \u201crefinement,\u201d \u201creduction\u201d), it has become crucial to develop alternative experimental models in cancer biology. Several studies have already described the avian chorioallantoic membrane (<jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content>) model as an alternative to rodents, suitable to investigate growth, progression and metastasis of various types of cancer. In the present work, we grafted three Merkel cell carcinoma (<jats:styled-content style=\"fixed-case\">MCC<\/jats:styled-content>) cell lines onto the avian <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> and monitored tumor growth and development of solid tumor nodules. Morphology of xenograft was characterised histologically and immunohistochemically. Our results demonstrate <jats:styled-content style=\"fixed-case\">CAM<\/jats:styled-content> assay as a useful tool to study <jats:styled-content style=\"fixed-case\">MCC<\/jats:styled-content> pathophysiology.<\/jats:p>","DOI":"10.1111\/exd.13529","type":"journal-article","created":{"date-parts":[[2018,3,6]],"date-time":"2018-03-06T17:22:43Z","timestamp":1520356963000},"page":"684-687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A short\u2010term in vivo model for Merkel Cell Carcinoma"],"prefix":"10.1111","volume":"27","author":[{"given":"Vishwanath Kumble","family":"Bhat","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Biochemistry Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinna","family":"Krump","sequence":"additional","affiliation":[{"name":"Institute of Pathophysiology and Immunology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Bernhart","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biochemistry Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen C.","family":"Becker","sequence":"additional","affiliation":[{"name":"Department for Translational Skin Cancer Research &amp; Department of Dermatology University Hospital Essen University of Duisburg\u2010Essen  Essen Germany"},{"name":"German Cancer Consortium (DKTK) Partner Site Essen\/D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Sattler","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biochemistry Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6696-8717","authenticated-orcid":false,"given":"Nassim","family":"Ghaffari\u2010Tabrizi\u2010Wizsy","sequence":"additional","affiliation":[{"name":"Institute of Pathophysiology and Immunology Medical University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2007.11.020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.198"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.currproblcancer.2010.01.001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.20167"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2016.10.022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/2000656X.2011.613233"},{"key":"e_1_2_9_8_1","author":"Colunga A.","year":"2017","journal-title":"Clin. 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