{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:52:20Z","timestamp":1740124340246,"version":"3.37.3"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methadone (Met) mainly acts as a \u03bc\u2010opioid receptor agonist. Recent evidence pointing towards the role of Met in sensitization of certain cancer cell lines to chemotherapeutic agents has promoted the hypothesis that Met may be a useful adjuvant to cancer chemotherapy. We wanted to address whether Met has, alone or in combination with a chemotherapeutic agent, an effect on melanoma cell viability in vitro. Only a small fraction (4.3%) of our 102 melanoma biobank cell lines with <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010sequencing data showed expression of the main receptor for Met (<jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1). We assessed the viability of melanoma cell lines with high, medium or low\/no <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1 expression (<jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>high<\/jats:sup>, <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>med<\/jats:sup>, <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>neg<\/jats:sup>) 72\u00a0hours after treatment with Met alone or combined with cisplatin (Cis). Our analyses show that Met alone did not affect cell viability. While Cis\/Met treatment did not have an effect on viability of <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>med<\/jats:sup> or <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>neg<\/jats:sup> cell lines, it resulted in a slightly decreased cell viability of <jats:styled-content style=\"fixed-case\">OPRM<\/jats:styled-content>1<jats:sup>high<\/jats:sup> cells. Clinically, concurrent temozolomide\/Met treatment did not have an effect in our single\u2010case report of a patient suffering from uveal melanoma. Taken together, our findings do not provide evidence for recommending Met as an adjuvant to chemotherapy in patients with melanoma.<\/jats:p>","DOI":"10.1111\/exd.13543","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T01:22:59Z","timestamp":1522027379000},"page":"694-696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Methadone\u2014Not a magic bullet in melanoma therapy"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8607-6254","authenticated-orcid":false,"given":"Marie\u2010Charlotte","family":"Br\u00fcggen","sequence":"first","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Mangana","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja","family":"Irmisch","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars E.","family":"French","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell P.","family":"Levesque","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phil F","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Dummer","sequence":"additional","affiliation":[{"name":"Department of Dermatology Zurich University Hospital  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,21]]},"reference":[{"issue":"7","key":"e_1_2_9_2_1","first-page":"CD012592","author":"Wiffen P. 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