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Epidermal cells are continuously replaced by turnover, and thus to understand in detail the dynamic cellular events in the epidermis, techniques to observe live tissues in 3D are required. Here, we established a live 3D imaging technique for epidermis models. We first obtained immortalized human epidermal cell lines which have a normal differentiation capacity and fluorescence\u2010labelled cytoplasm or nuclei. The reconstituted 3D epidermis was prepared with these lines. Using this culture system, we were able to observe the structure of the reconstituted epidermis live in 3D, which was similar to an in vivo epidermis, and evaluate the effect of a skin irritant. This technique may be useful for dermatological science and drug development.<\/jats:p>","DOI":"10.1111\/exd.13672","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:02:09Z","timestamp":1524787329000},"page":"563-570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of 3D imaging technique of reconstructed human epidermis with immortalized human epidermal cell line"],"prefix":"10.1111","volume":"27","author":[{"given":"Yu","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Cellular Biophysics Graduate School of Pharmaceutical Sciences Nagoya City University  Nagoya Aichi Japan"},{"name":"Research Laboratories Nippon Menard Cosmetic Co., Ltd.  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