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Dissociated epidermal and dermal progenitor cells, isolated either from neonatal C57BL\/6 mice or human foetal scalp tissues, were suspended in (10\u00a0\u03bcL) F12 medium and pipetted into a 1 or 2\u00a0mm\u2010diameter punch biopsy wounds on the back skin of immunodeficient mice. At 3\u00a0weeks after transplantation, although pigmented mouse hairs could efficiently form at the injection sites with delivery of mouse cells, none hair formed on the host mouse skin at 3\u00a0months after delivery of human cells. Under the same conditions, human follicles could be regenerated when the human skin cells were delivered onto a 2\u00a0mm size punch created on a reconstituted human skin (hRSK), which previously generated on the back of an immunodeficient mouse, but the efficiency of hair formation was low. We demonstrated that both mouse and human regenerated follicles showed normal histology and differentiation markers; moreover, the cell chasing experiment confirmed that the regenerated hair follicles were formed from transplanted cells. Compared to other current hair reconstituted assays, the Punch Assay is relatively simple and generates normal hair follicles within a smaller wound. We suggest that the punch assay is a better in vivo assay of cell trichogenicity.<\/jats:p>","DOI":"10.1111\/exd.13753","type":"journal-article","created":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T06:36:06Z","timestamp":1532327766000},"page":"349-356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Dissociated skin cells regenerate hair follicles in a microwound, \u201cThe Punch Assay\u201d"],"prefix":"10.1111","volume":"29","author":[{"given":"Xue","family":"Leng","sequence":"first","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Out\u2010patient Surgery Qilu Hospital Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urinary Surgery Qilu Children's Hospital of Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dingyang","family":"Li","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Wen","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Qian","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0822-1700","authenticated-orcid":false,"given":"Xunwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Laboratory for Tissue Engineering and Regeneration School of Stomatology Shandong University  Jinan Shandong China"},{"name":"Suzhou Institute of Shandong University  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2015.07.003"},{"key":"e_1_2_8_3_1","first-page":"186","volume":"45","author":"Clarke P.","year":"2016","journal-title":"Aust. 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