{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:56:08Z","timestamp":1790661368940,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["67\/19\/2013"],"award-info":[{"award-number":["67\/19\/2013"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytokines regulate immune response and inflammation and play an important role in depigmentation process of an autoimmune disease, vitiligo. We sought to determine how inflammatory cytokines influence the progression of vitiligo, and based on that, we develop a logical therapeutic intervention using primary melanocyte culture. Melanocytes were cultured and exposed to <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2, <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 for 4\u00a0days. Melanocytes proliferation, tyrosinase assay and melanin content were measured. Real\u2010Time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was used to analyse <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of genes specific for melanocytes growth and pigmentation. Anti\u2010<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A receptor antibody was used to block <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A receptors expressed on melanocytes. Protein expression of <jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TYR<\/jats:styled-content> was assessed by immunofluorescence and Western blotting. A gradual decline in the melanocyte population, melanin content and tyrosinase activity was observed after different cytokine treatment. The expression of <jats:italic><jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content><\/jats:italic> and its downstream genes after blocking with anti\u2010<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>, an increased melanin content, increased expression of <jats:styled-content style=\"fixed-case\">TYR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MITF<\/jats:styled-content> along with its downstream genes, and cell proliferation was observed.<\/jats:p>","DOI":"10.1111\/exd.13773","type":"journal-article","created":{"date-parts":[[2018,8,18]],"date-time":"2018-08-18T08:44:43Z","timestamp":1534581883000},"page":"682-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Role of <scp>IL<\/scp>\u201017A receptor blocking in melanocyte survival: A strategic intervention against vitiligo"],"prefix":"10.1111","volume":"28","author":[{"given":"Supriya","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Departmet of Dermatology Postgraduate Institute of Medical Education &amp; Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alka","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine &amp; Biotechnology Postgraduate Institute of Medical Education &amp; Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthu Sendhil","family":"Kumaran","sequence":"additional","affiliation":[{"name":"Departmet of Dermatology Postgraduate Institute of Medical Education &amp; Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0348-0815","authenticated-orcid":false,"given":"Davinder","family":"Parsad","sequence":"additional","affiliation":[{"name":"Departmet of Dermatology Postgraduate Institute of Medical Education &amp; Research  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1977.01640010049006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2008.09.048"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2002.1o049.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08785.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2010.03972.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2016.12.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2006.07.008"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.237"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110496"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22010134"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014241108"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3908061"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700125"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3680"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2011.00939.x"},{"key":"e_1_2_9_18_1","first-page":"849","volume":"24","author":"Moretti S.","year":"2009","journal-title":"Histol. 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