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It is the aim of our study to analyse the distribution of the pro\u2010apoptotic transcription factors FoxO1 and FoxO3 in the nuclear and cytoplasmic compartments of human sebocytes in vivo before and during isotretinoin treatment of acne patients. Immunohistochemical analysis of skin biopsies with antibodies distinguishing phosphorylated and non\u2010phosphorylated human FoxO1 and FoxO3 proteins was performed before isotretinoin treatment, six weeks after initiation of isotretinoin therapy, and in acne\u2010free control patients not treated with isotretinoin. Our in vivo study demonstrates a significant increase in the nucleo\u2010cytoplasmic ratio of non\u2010phosphorylated FoxO1 and FoxO3 during isotretinoin treatment of acne patients. Translational and presented experimental evidence indicates that upregulation of nuclear FoxO1 and FoxO3 proteins is involved in isotretinoin\u2010induced pro\u2010apoptotic signalling in sebocytes confirming the scientific hypothesis of isotretinoin\u2010mediated upregulation of FoxO expression.<\/jats:p>","DOI":"10.1111\/exd.13787","type":"journal-article","created":{"date-parts":[[2018,9,22]],"date-time":"2018-09-22T02:14:55Z","timestamp":1537582495000},"page":"1344-1351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Effect of oral isotretinoin on the nucleo\u2010cytoplasmic distribution of FoxO1 and FoxO3 proteins in sebaceous glands of patients with acne vulgaris"],"prefix":"10.1111","volume":"27","author":[{"given":"Naglaa Fathi","family":"Agamia","sequence":"first","affiliation":[{"name":"Department of Dermatology, Andrology and Venereology Faculty of Medicine Alexandria University  Alexandria 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