{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:23:05Z","timestamp":1787163785877,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SCHR 1288\/6\u20101"],"award-info":[{"award-number":["SCHR 1288\/6\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n In humans, there are four known proton\u2010sensing G\u2010Protein\u2010coupled receptors (pH\u2010GPCRs): GPR4 (GPR19), TDAG8 (GPR65, T\u2010cell death\u2010associated gene 8), OGR1 (GPR68, ovarian cancer GPCR1) and G2A (GPR132, G2 accumulation protein). They are known to be involved in sensing changes of extracellular proton concentrations in the acidic microenvironment of tumors, which leads to altered cell proliferation, migration, metastasis, immune cell function and inflammation. However, little is known about the expression of pH\u2010GPCRs in the skin and especially skin cancers.<\/jats:p>\n <\/jats:sec>\n \n Aim<\/jats:title>\n \n We studied the expression of pH\u2010GPCRs in selected skin cancers, that is Merkel cell carcinoma (\n MCC<\/jats:styled-content>\n ), dermatofibrosarcoma protuberans (\n DFSP<\/jats:styled-content>\n ), atypical fibroxanthoma (\n AFX<\/jats:styled-content>\n ) and pleomorphic dermal sarcoma (\n PDS<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We did immunohistochemistry and immunofluorescence to analyse the expression of GPR4, TDAG8, OGR1 and G2A using paraffin\u2010embedded tissue samples (n\u00a0=\u00a04, exceptions:\n PDS<\/jats:styled-content>\n GPR4\/GPR65 n\u00a0=\u00a05,\n AFX<\/jats:styled-content>\n GPR132 n\u00a0=\u00a03) from patients suffering from\n MCC<\/jats:styled-content>\n ,\n DFSP<\/jats:styled-content>\n ,\n AFX<\/jats:styled-content>\n and\n PDS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n (a) GPR4 was expressed on all\n AFX<\/jats:styled-content>\n and\n PDS<\/jats:styled-content>\n specimens. All\n AFX<\/jats:styled-content>\n and\n MCC<\/jats:styled-content>\n showed a positive expression of G2A. All\n PDS<\/jats:styled-content>\n exhibited a strong positive expression of G2A. (b)\n MCC<\/jats:styled-content>\n s neither expressed GPR4 nor TDAG8. All\n DFSP<\/jats:styled-content>\n showed no expression of TDAG8. (c) For any other combination of\n GPCR<\/jats:styled-content>\n and skin disease, we found positive\/negative mixed results.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n These are the first results on pH\u2010GPCRs in selected skin cancers. We provide evidence that these GPCRs are differentially expressed on the various types of skin cancers and that they can potentially be addressed as a therapeutic target in extensive disease.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/exd.13809","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T11:11:33Z","timestamp":1539947493000},"page":"66-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Expression of proton\u2010sensing G\u2010protein\u2010coupled receptors in selected skin tumors"],"prefix":"10.1111","volume":"28","author":[{"given":"Ana\u00efs","family":"Nassios","sequence":"first","affiliation":[{"name":"Department of Dermatology University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Wallner","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Haferkamp","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Klingelh\u00f6ffer","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Brochhausen","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2820-1942","authenticated-orcid":false,"given":"Stephan","family":"Schreml","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmb.20952"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01649_3.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13209"},{"key":"e_1_2_7_5_1","volume-title":"Biochemie und Pathobiochemie","author":"L\u00f6ffler G.","year":"2006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.90.101"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2005.06.002"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3554"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3110"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1713"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.12.006"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00370"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00354"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01905"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01909-06"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803466"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.08.013"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027586"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10456-011-9238-9"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djm107"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.10.122"},{"key":"e_1_2_7_22_1","first-page":"1327","volume":"124","author":"Ren J.","year":"2011","journal-title":"Chin. Med. J."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061991"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001165107"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408040200"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12334"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13809","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13809","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.13809","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13809","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T09:28:01Z","timestamp":1694683681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,13]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/exd.13809"],"URL":"https:\/\/doi.org\/10.1111\/exd.13809","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,13]]},"assertion":[{"value":"2018-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}