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The current study aims to use reconstructed human skin equivalents to demonstrate topical stressor\u2010induced hyperpigmentation and evaluate bioactives\u2019 potential protective effect. Given that polycyclic aromatic hydrocarbons are representative airborne particle\u2010bound organic compounds with known relevance to pigmentation pathways, benzo(a)pyrene was selected as surrogate environmental toxin. On the other hand, squalene monohydroperoxides are well\u2010characterized sebum peroxidation product under <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> and pollutant exposure, thus are used as another representative skin stressor. With 3\u2010day continuous exposure, 30\u00a0pmol\/cm<jats:sup>2<\/jats:sup> of benzo(a)pyrene and 3.4\u00a0nmol\/cm<jats:sup>2<\/jats:sup> of squalene monohydroperoxides induced significant viability loss, inflammatory response, and approximately 10 shades of pigmentation increase in pigmented living skin equivalents. At the same time, pretreatment and co\u2010treatment with 12\u2010hydroxystearic acid (12\u2010<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content>, 20\u00a0\u03bcmol\/L) or niacinamide (5\u00a0mmol\/L) ameliorated such stressor\u2010induced consequences. Niacinamide was particularly effective against benzo(a)pyrene damage, probably as a substrate for important <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content>+ dependent detoxification pathways, while 12\u2010<jats:styled-content style=\"fixed-case\">HSA<\/jats:styled-content> was potent against squalene monohydroperoxides through barrier enhancing, anti\u2010inflammatory, and anti\u2010oxidative mechanisms. In summary, topical stressor\u2010induced hyperpigmentation was achieved in vitro, with known bioactives showing protective benefits.<\/jats:p>","DOI":"10.1111\/exd.13811","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T16:07:35Z","timestamp":1539965255000},"page":"742-746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Niacinamide and 12\u2010hydroxystearic acid prevented benzo(a)pyrene and squalene peroxides induced hyperpigmentation in skin equivalent"],"prefix":"10.1111","volume":"28","author":[{"given":"Tingyan","family":"Mi","sequence":"first","affiliation":[{"name":"Unilever R&amp;D Shanghai  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiying","family":"Dong","sequence":"additional","affiliation":[{"name":"Unilever R&amp;D Shanghai  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uma","family":"Santhanam","sequence":"additional","affiliation":[{"name":"Unilever R&amp;D Trumbull  Trumbull Connecticut"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7873-7294","authenticated-orcid":false,"given":"Nan","family":"Huang","sequence":"additional","affiliation":[{"name":"Unilever R&amp;D Shanghai  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2015.12.045"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12270"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.204"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3614"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2010.10.010"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2015.01.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2017.01.007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2016.07.026"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.6b00364"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.03.039"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atmosres.2014.06.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-016-1623-1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12208"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.32.1504"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04834.x"},{"issue":"1","key":"e_1_2_8_17_1","first-page":"AB184","volume":"68","author":"Hawkins S.","year":"2013","journal-title":"J. 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