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There have been only a few attempts to treat alopecia areata (<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>) with <jats:styled-content style=\"fixed-case\">MSCT<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">MSCT<\/jats:styled-content> efficacy and mechanism of action in treating <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content> are not known. We sought to investigate the effect of human hematopoietic mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s) on an in vitro model of <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content> and to explore relevant mechanisms that regulate efficacy. An <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>\u2010like environment was induced by pretreatment of human dermal papilla cells (<jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s) with interferon gamma (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3). <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s were administered to the <jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s, and cell viability was determined. Similar studies were also conducted with human hair follicles (HFs) in culture. The change in expression of the Wnt\/\u03b2\u2010catenin pathway and <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content> pathway\u2010related molecules and growth factors in <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>\u2010treated <jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s was also examined by reverse transcription\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Western blot assay and growth factor array. Immune privilege\u2013related molecules were examined by immunohistochemistry in HF culture models. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s enhanced the cell viability of the <jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s activated several molecules in the Wnt\/\u03b2\u2010catenin signalling pathway, including \u00df\u2010catenin and phosphorylated <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>3b, and decreased <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3\u2010induced expression of <jats:styled-content style=\"fixed-case\">DKK<\/jats:styled-content>1 in <jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s suppressed <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3\u2010induced expression of caspase\u20101, caspase\u20103 and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 receptor. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>s induced the phosphorylation of <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 compared to controls and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3\u2013pretreated <jats:styled-content style=\"fixed-case\">hDPC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content>\u2010treated HFs enhanced several growth factor <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">hHMSC<\/jats:styled-content> pretreatment modulated <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3\u2013induced expression of molecules related to HF immune privilege on HFs in organ culture. These data suggest <jats:styled-content style=\"fixed-case\">MSCT<\/jats:styled-content> may be a new potential therapeutic option in treating AA.<\/jats:p>","DOI":"10.1111\/exd.13812","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T21:00:54Z","timestamp":1540846854000},"page":"265-272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Effects of mesenchymal stem cell therapy on alopecia areata in cellular and hair follicle organ culture models"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1670-0995","authenticated-orcid":false,"given":"Jung Eun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jee Hye","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Jun","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hee","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3631-0866","authenticated-orcid":false,"given":"Kwan Ho","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9822-4760","authenticated-orcid":false,"given":"Hoon","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Dermatology St. Paul's Hospital College of Medicine The Catholic University of Korea  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-11-785865"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2401"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2017.12.009"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00645"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2012.02.004"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2437"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2018.02.003"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/394917"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.368"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.180"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12209"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2014-207201"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvv069"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-015-0331-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2017.1365111"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13255"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12827"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.08.029"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/311560a0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1560-4"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1500973"},{"key":"e_1_2_8_23_1","first-page":"43","volume":"18","author":"Oliver R. 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