{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:19:14Z","timestamp":1788581954265,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T00:00:00Z","timestamp":1548806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"Abstract<\/jats:title>\n \n Carbonylated proteins (\n CP<\/jats:styled-content>\n s) are synthesized by reactions between amino groups in proteins and reactive aldehyde compounds (\n RAC<\/jats:styled-content>\n ) yielded from lipid peroxidation initiated by reactive oxygen species (\n ROS<\/jats:styled-content>\n ). In the skin,\n CP<\/jats:styled-content>\n s are detected in a higher frequency at sun\u2010exposed sites of the skin in elderly subjects. Since\n CP<\/jats:styled-content>\n s in the stratum corneum (\n SC<\/jats:styled-content>\n ) have been reported to correlate with skin water content and transepidermal water loss, it is considered that the accumulation of\n CP<\/jats:styled-content>\n s in the\n SC<\/jats:styled-content>\n involves the loss of skin moisture functions. However, the roles of\n CP<\/jats:styled-content>\n s in the dermis on skin physiology are still unclear. The purpose of this study was to investigate the roles of\n CP<\/jats:styled-content>\n s in the dermis during the progression of photoaged skin and to propose a method to prevent or reduce the synthesis of\n CP<\/jats:styled-content>\n s. The exposure of human normal dermal fibroblasts to\n CP<\/jats:styled-content>\n s increased intracellular\n ROS<\/jats:styled-content>\n levels and the synthesis of intracellular\n CP<\/jats:styled-content>\n s. In addition,\n CP<\/jats:styled-content>\n s caused morphological changes of fibroblasts. Furthermore,\n CP<\/jats:styled-content>\n s caused alterations of\n mRNA<\/jats:styled-content>\n expression levels of dermal matrix\u2010related proteins, such as upregulating\n MMP<\/jats:styled-content>\n \u20101 and\n IL<\/jats:styled-content>\n \u20108. These results indicated that\n CP<\/jats:styled-content>\n s disrupt construction of the dermal matrix. On the other hand, \u03b1\u2010tocopherol and \u03b2\u2010carotene suppressed the synthesis of\n RAC<\/jats:styled-content>\n during lipid peroxidation which resulted in the reduction of\n UVA<\/jats:styled-content>\n \u2010induced\n CP<\/jats:styled-content>\n s in the\n SC<\/jats:styled-content>\n . From these results, we propose that extracellular\n CP<\/jats:styled-content>\n s increase intracellular\n ROS<\/jats:styled-content>\n levels and contribute to alterations of the dermal matrix. To prevent the synthesis of\n CP<\/jats:styled-content>\n s, the application of \u03b1\u2010tocopherol or \u03b2\u2010carotene could be effective.\n <\/jats:p>","DOI":"10.1111\/exd.13821","type":"journal-article","created":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T13:16:27Z","timestamp":1548854187000},"page":"32-37","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The impact of carbonylated proteins on the skin and potential agents to block their effects"],"prefix":"10.1111","volume":"28","author":[{"given":"Yumiko","family":"Yamawaki","sequence":"first","affiliation":[{"name":"School of Bioscience and Biotechnology Tokyo University of Technology Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taeko","family":"Mizutani","sequence":"additional","affiliation":[{"name":"School of Bioscience and Biotechnology Tokyo University of Technology Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Okano","sequence":"additional","affiliation":[{"name":"School of Bioscience and Biotechnology Tokyo University of Technology Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Masaki","sequence":"additional","affiliation":[{"name":"School of Bioscience and Biotechnology Tokyo University of Technology Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2004.06.010"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700960"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12323724"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707395"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2011.06.015"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2016.09.016"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-2494.2008.00422.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12392"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2007.00195.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1979.tb07052.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2009.00396.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2001.280.5.E685"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.334"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.74.1.267"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0570"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2007.11.010"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00164"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2010.502982"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072563"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2004.031"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060128"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.01.008"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13821","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13821","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T00:36:46Z","timestamp":1694479006000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,30]]},"references-count":22,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/exd.13821"],"URL":"https:\/\/doi.org\/10.1111\/exd.13821","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,30]]},"assertion":[{"value":"2018-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}