{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:31:30Z","timestamp":1761060690907,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T00:00:00Z","timestamp":1547510400000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The role of epidermal proteolysis in overdesquamation was revealed in Netherton syndrome, a rare ichthyosis due to genetic deficiency of the LEKTI inhibitor of serine proteases. Recently, we developed activography, a new histochemical method, to spatially localize and semiquantitatively assess proteolytic activities using activity\u2010based probes. Activography provides specificity and versatility compared to in situ zymography, the only available method to determine enzymatic activities in tissue biopsies. Here, activography was validated in skin biopsies obtained from an array of distinct disorders and compared with in situ zymography. Activography provides a methodological advancement due to its simplicity and specificity and can be readily adapted as a routine diagnostic assay. Interestingly, the levels of epidermal proteolysis correlated with the degree of desquamation independent of skin pathology. Thus, deregulated epidermal proteolysis likely represents a universal mechanism underlying aberrant desquamation.<\/jats:p>","DOI":"10.1111\/exd.13832","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T12:19:47Z","timestamp":1541247587000},"page":"86-89","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Activography reveals aberrant proteolysis in desquamating diseases of differing backgrounds"],"prefix":"10.1111","volume":"28","author":[{"given":"Eleni","family":"Zingkou","sequence":"first","affiliation":[{"name":"Department of Pharmacy School of Health Sciences University of Patras  Rion\u2010Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgios","family":"Pampalakis","sequence":"additional","affiliation":[{"name":"Department of Pharmacy School of Health Sciences University of Patras  Rion\u2010Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitra","family":"Kiritsi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Faculty of Medicine Medical Center\u2010University of Freiburg  Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manthoula","family":"Valari","sequence":"additional","affiliation":[{"name":"Aghia Sofia Children's Hospital  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Jonca","sequence":"additional","affiliation":[{"name":"Epithelial Differentiation and Rheumatoid Autoimmunity Unit (UDEAR) H\u00f4pital Purpan UMR 1056 Inserm \u2010 Universit\u00e9 de Toulouse  Toulouse Cedex 9 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-4914","authenticated-orcid":false,"given":"Georgia","family":"Sotiropoulou","sequence":"additional","affiliation":[{"name":"Department of Pharmacy School of Health Sciences University of Patras  Rion\u2010Patras Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3175"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.11.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2012.09.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608348200"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7CC01081H"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005389"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1493"},{"key":"e_1_2_8_9_1","first-page":"137","volume":"2017","author":"Pampalakis G.","year":"1808","journal-title":"J. Invest. Dermatol."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.01.025"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2007.00562.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.11.009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13111"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.004"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471140864.ps2117s36"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/75977"},{"key":"e_1_2_8_17_1","volume":"3","author":"Borda L. J.","year":"2015","journal-title":"J. Clin. Investig. Dermatol."},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.23"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.10.004"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.11.009"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20952"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09582.x"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13832","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13832","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T13:27:50Z","timestamp":1694698070000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13832"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/exd.13832"],"URL":"https:\/\/doi.org\/10.1111\/exd.13832","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"type":"print","value":"0906-6705"},{"type":"electronic","value":"1600-0625"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"2018-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}