{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:02:04Z","timestamp":1792036924979,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T00:00:00Z","timestamp":1549843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"PRIN","award":["Prot-2012-JJX494"],"award-info":[{"award-number":["Prot-2012-JJX494"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cutaneous melanoma (CM) is one of the most prevalent skin cancers, which lacks both a prognostic marker and a specific and lasting treatment, due to the complexity of the disease and heterogeneity of patients. Reflectance confocal microscopy (RCM) in vivo analysis is a versatile approach offering immediate morphological information, enabling the identification of four primary cutaneous RCM CM types. Whether RCM CM types are associated with a specific protein and molecular genetic profiles at the tissue level remains unclear. The current pilot study was designed to identify potential correlations between RCM CM types and specific biological characteristics, combining immunohistochemistry (IHC) and molecular analyses. Eighty primary CMs evaluated at patient bedside with RCM (type 1 [19, 24%], type 2 [12, 15%], type 3 [7, 9%] and type 4 [42, 52%]) were retrospectively evaluated by IHC stains (CD271, CD20, CD31, cyclin D1), fluorescence in situ hybridization FISH for MYC gain and CDKN2A loss and molecular analysis for somatic mutations (BRAF, NRAS and KIT). RCM CM types correlated with markers of stemness property, density of intra\u2010tumoral lymphocytic B infiltrate and cyclin D1 expression, while no significant association was found with blood vessel density nor molecular findings. RCM CM types show a different marker profile expression, suggestive of a progression and an increase in aggressiveness, according to RCM morphologies.<\/jats:p>","DOI":"10.1111\/exd.13877","type":"journal-article","created":{"date-parts":[[2019,1,12]],"date-time":"2019-01-12T13:54:43Z","timestamp":1547301283000},"page":"254-260","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Melanoma types by in vivo reflectance confocal microscopy correlated with protein and molecular genetic alterations: A\u00a0pilot study"],"prefix":"10.1111","volume":"28","author":[{"given":"Francesca","family":"Beretti","sequence":"first","affiliation":[{"name":"Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8994-8791","authenticated-orcid":false,"given":"Laura","family":"Bertoni","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7088-9077","authenticated-orcid":false,"given":"Francesca","family":"Farnetani","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine University of Modena and Reggio Emilia  Modena Italy"},{"name":"Department of Dermatology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L'Aquila  L'Aquila Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greta","family":"Gorelli","sequence":"additional","affiliation":[{"name":"Division of Pathological Anatomy Department of Surgery and Translational Medicine University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Cesinaro","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Azienda Ospedaliero\u2010Universitaria Policlinico  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Reggiani Bonetti","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences of Children &amp; Adults University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-7526","authenticated-orcid":false,"given":"Lucia","family":"Di Nardo","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L'Aquila  L'Aquila Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaniko","family":"Kaleci","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Chester","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Longo","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Modena and Reggio Emilia  Modena Italy"},{"name":"Azienda Unit\u00e0 Sanitaria Locale\u2010IRCCS di Reggio Emilia Centro Oncologico ad Alta Tecnologia Diagnostica  Reggio Emilia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Massi","sequence":"additional","affiliation":[{"name":"Division of Pathological Anatomy Department of Surgery and Translational Medicine University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C.","family":"Fargnoli","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L'Aquila  L'Aquila Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Pellacani","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine University of Modena and Reggio Emilia  Modena Italy"},{"name":"Department of Dermatology University of Modena and Reggio Emilia  Modena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2016.88"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-018-9892-z"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16112"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06489"},{"key":"e_1_2_7_6_1","first-page":"130","volume":"2010","author":"Perego M.","year":"1877","journal-title":"J. Invest. Dermatol."},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09161"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-10129-z"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2016.03.022"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2015.12.001"},{"key":"e_1_2_7_11_1","first-page":"3200","volume":"62","author":"Sauter E. R.","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2011.02.011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63536-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.5858\/2011-0048-RAIR.1","volume":"135","author":"Gerami P.","year":"2011","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000380"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2017.10.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.37"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31823425cc"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.193"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.228"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16364"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12417"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12210"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000147"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179745"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13301"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2016.1189"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000323"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.15.24"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.028860"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12437"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20016"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2011.01.011"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2017.08.006"},{"key":"e_1_2_7_35_1","first-page":"3511","volume":"6","author":"Cachia A. R.","year":"2000","journal-title":"Clin. Cancer Res."},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20016"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-006-0167-8"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-006-0150-4"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1535"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.14028"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13877","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13877","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T10:40:28Z","timestamp":1694428828000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13877"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,11]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1111\/exd.13877"],"URL":"https:\/\/doi.org\/10.1111\/exd.13877","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,11]]},"assertion":[{"value":"2018-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}