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The current pharmacological management of this condition is mostly based on small molecule drugs predominately effective in ameliorating the inflammatory condition. Emerging molecular approaches could present an opportunity for managing rosacea conditions at transcriptomic level, and in the future allow personalized approaches. <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> medicines, such as small <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference (siRNA), could provide a flexible and applicable tool reaching this aim. However, the topical si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> delivery by dermatological emulsions, commonly used in the daily management of rosacea, is still largely unexplored. Consequently, <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference application to rosacea was defined on molecular bases by genetic expression meta\u2010data analysis. Based on this, a si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> directed against <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>2 was designed and validated in vitro on murine macrophages and fibroblasts. Next, si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was dispersed in the continuous phase of emulsions and was characterized for commonly used dermatologic bases. Finally, the potential delivery performance of the topical emulsions was tested in vivo on healthy Balb\/c mice. It was found that the interaction of si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> with combination of excipients, such as urea and glycerol, is likely to favour the si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> delivery, inducing genetic silencing of <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>2. These findings provide a foundation for the future development of topical <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010based dispersions for topical molecular medicines, by emphasizing on the formulation and therapeutic\u2010based opportunities with dermatological treatments.<\/jats:p>","DOI":"10.1111\/exd.13881","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T20:21:22Z","timestamp":1547670082000},"page":"261-269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring the potential for rosacea therapeutics of si<scp>RNA<\/scp> dispersion in topical emulsions"],"prefix":"10.1111","volume":"28","author":[{"given":"Stefano","family":"Colombo","sequence":"first","affiliation":[{"name":"Umbed Pharmaceuticals  Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Necati","family":"Harmankaya","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorrit J.","family":"Water","sequence":"additional","affiliation":[{"name":"Umbed Pharmaceuticals  Frederiksberg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4151-4097","authenticated-orcid":false,"given":"Adam","family":"Bohr","sequence":"additional","affiliation":[{"name":"Umbed Pharmaceuticals  Frederiksberg Denmark"},{"name":"Department of Pharmacy Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2220"},{"key":"e_1_2_9_3_1","unstructured":"Rosacea support group. 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