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We investigated the effects of baicalein and sulphoraphane on ultraviolet B (UVB<\/jats:styled-content>) irradiation\u2013inducedMMP<\/jats:styled-content>\u20101 expression and apoptosis using human dermal fibroblasts.UVB<\/jats:styled-content>irradiation not only increasedMMP<\/jats:styled-content>\u20101 expression, but also caused apoptosis. Both baicalein and sulphoraphane protected cells fromUVB<\/jats:styled-content>irradiation\u2013induced apoptosis, but only baicalein inhibitedMMP<\/jats:styled-content>\u20101 expression.UVB<\/jats:styled-content>irradiation activated 12\u2010lipoxygenase, and its product, 12\u2010hydroxyeicosatetraenoic acid, activatedTRPV<\/jats:styled-content>1 channels. The resultingUVB<\/jats:styled-content>irradiation\u2013induced Ca2+<\/jats:sup>increase was blocked by the 12\u2010lipoxygenase inhibitor baicalein and theTRPV<\/jats:styled-content>1 blocker capsazepine, but not by the Nrf2 inducer sulphoraphane.UVB<\/jats:styled-content>irradiation also increasedROS<\/jats:styled-content>generation and decreased Nrf2 protein levels.UVB<\/jats:styled-content>irradiation\u2013inducedMMP<\/jats:styled-content>\u20101 expression was blocked by the Ca2+<\/jats:sup>chelatorBAPTA<\/jats:styled-content>, by capsazepine and byTRPV<\/jats:styled-content>1 silencing. However, induction was unaffected by the antioxidant N\u2010acetylcysteine.ERK<\/jats:styled-content>phosphorylation andJNK<\/jats:styled-content>phosphorylation were induced byUVB<\/jats:styled-content>irradiation, but onlyERK<\/jats:styled-content>phosphorylation was Ca2+<\/jats:sup>sensitive. IncreasedMMP<\/jats:styled-content>\u20101 expression was blocked byPD<\/jats:styled-content>98059, but not bySP<\/jats:styled-content>600125. Thus, increasedMMP<\/jats:styled-content>\u20101 expression is mediated by increased cytosolic Ca2+<\/jats:sup>andERK<\/jats:styled-content>phosphorylation.UVB<\/jats:styled-content>irradiation\u2013inducedROS<\/jats:styled-content>generation is also Ca2+<\/jats:sup>sensitive, andUVB<\/jats:styled-content>irradiation\u2013induced apoptosis is caused by increasedROS<\/jats:styled-content>. Thus, baicalein, by blocking theUVB<\/jats:styled-content>irradiation\u2013induced cytosolic Ca2+<\/jats:sup>increase, protects cells fromUVB<\/jats:styled-content>irradiation\u2013inducedMMP<\/jats:styled-content>\u20101 expression and apoptosis. In contrast, sulphoraphane, by decreasing cellularROS<\/jats:styled-content>, protects cells from onlyUVB<\/jats:styled-content>\u2010induced apoptosis. Thus, targeting 12\u2010lipoxygenase may provide a therapeutic approach to improving the health of photo\u2010aged human skin.<\/jats:p>","DOI":"10.1111\/exd.13912","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T11:26:10Z","timestamp":1551525970000},"page":"568-575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Baicalein inhibits matrix metalloproteinase 1 expression via activation ofTRPV<\/scp>1\u2010Ca\u2010ERK<\/scp>pathway in ultraviolet B\u2013irradiated human dermal fibroblasts"],"prefix":"10.1111","volume":"28","author":[{"given":"Kuo\u2010Feng","family":"Huang","sequence":"first","affiliation":[{"name":"Division of Plastic Surgery Department of Surgery Chi Mei Medical Center Tainan Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo\u2010Hsing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biology and Anatomy National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen\u2010Jung","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang\u2010Chuan","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010You","family":"Jhap","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Hua","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology Soochow University Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9741-3017","authenticated-orcid":false,"given":"Sheau\u2010Huei","family":"Chueh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry National Defense Medical Center Taipei Taiwan Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"M. 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