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To reveal dynamic epigenetic and transcriptome changes of keloid fibroblasts, we performed <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq and <jats:styled-content style=\"fixed-case\">ATAC<\/jats:styled-content>\u2010seq analysis on an early passage keloid fibroblast cell strain and its paired normal control fibroblasts. This keloid strain produced keloid\u2010like scars in a plasma clot\u2010based skin equivalent humanized keloid animal model. <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq analysis reveals gene ontology terms including hepatic fibrosis, Wnt\u2010\u03b2\u2010catenin, <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2, regulation of epithelial\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 and adherens junction. <jats:styled-content style=\"fixed-case\">ATAC<\/jats:styled-content>\u2010seq analysis suggests <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 signalling is the most significantly enriched gene ontology term in keloid fibroblasts, followed by Wnt signalling (Wnt5) and regulation of the <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> pathway. Immunohistochemistry confirms that <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 (Tyr705 phospho\u2010<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3) is activated and \u03b2<jats:bold>\u2010<\/jats:bold>catenin is up\u2010regulated in the dermis of keloid clinical specimens and keloid skin equivalent implants from the humanized mouse model. A non\u2010linear dose\u2010response of cucurbitacin I, a selective <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>2\/<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 inhibitor, in collagen type I expression of keloid\u2010derived plasma clot\u2010based skin equivalents implicates a likely role of <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>3 signalling in keloid pathogenesis. This work also demonstrates the utility of the recently established humanized keloid mouse model in exploring the mechanism of keloid formation.<\/jats:p>","DOI":"10.1111\/exd.13923","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:21:03Z","timestamp":1553732463000},"page":"480-484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["<scp>STAT<\/scp>3 signalling pathway is implicated in keloid pathogenesis by preliminary transcriptome and open chromatin analyses"],"prefix":"10.1111","volume":"28","author":[{"given":"Yun\u2010Shain","family":"Lee","sequence":"first","affiliation":[{"name":"The Saban Research Institute of Children's Hospital Los Angeles Los Angeles California"},{"name":"Integrative Stem Cell Center China Medical University Hospital China Medical University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Chen","family":"Liang","sequence":"additional","affiliation":[{"name":"Integrative Stem Cell Center China Medical University Hospital China Medical University Taichung Taiwan"},{"name":"Department of Pathology Keck School of Medicine University of Southern California Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Keck School of Medicine University of Southern California Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Kulber","sequence":"additional","affiliation":[{"name":"Plastic and Reconstructive Surgery Department of Surgery Keck School of Medicine University of Southern California Los Angeles California"},{"name":"Department of Orthopedic Surgery Cedars Sinai Medical Center Los Angeles California"},{"name":"The Plastic Surgery Center of Excellence Cedars Sinai Medical Center Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kylie","family":"Tanabe","sequence":"additional","affiliation":[{"name":"The Plastic Surgery Center of Excellence Cedars Sinai Medical Center Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Ming","family":"Chuong","sequence":"additional","affiliation":[{"name":"Integrative Stem Cell Center China Medical University Hospital China Medical University Taichung Taiwan"},{"name":"Department of Pathology Keck School of Medicine University of Southern California Los Angeles California"},{"name":"iWRR International laboratory for Wound Repair and Regenerative Research Graduated Institute of Clinical Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6603-4823","authenticated-orcid":false,"given":"Randall","family":"Widelitz","sequence":"additional","affiliation":[{"name":"Department of Pathology Keck School of Medicine University of Southern California Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7746-4677","authenticated-orcid":false,"given":"Tai\u2010Lan","family":"Tuan","sequence":"additional","affiliation":[{"name":"The Saban Research Institute of Children's Hospital Los Angeles Los Angeles California"},{"name":"Department of Surgery Keck School of Medicine University of Southern California Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2013.826651"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00553.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(97)80541-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-014-1490-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jisp.2016.10.024"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2498"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jccw.2016.10.001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.21017"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.11190.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000231899"},{"key":"e_1_2_9_12_1","first-page":"1033","volume":"30","author":"Jumper N.","year":"2015","journal-title":"Histol. 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