{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T05:18:08Z","timestamp":1792300688826,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T00:00:00Z","timestamp":1557792000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Defects in keratinocyte transglutaminase (<jats:styled-content style=\"fixed-case\">TGM<\/jats:styled-content>1), resulting in an improper protein scaffold for deposition of the lipid barrier, comprise a major source of autosomal recessive congenital ichthyosis. For that reason, the composition and formation of the cornified (cross\u2010linked) protein envelope of the epidermis have been of considerable interest. Since the isopeptide cross\u2010linked protein components are not individually isolable once incorporated, purified envelopes were analysed by mass spectrometry after trypsin digestion. Quantitative estimates of the identified components revealed some 170 proteins, each comprising at least 0.001% of the total, of which keratins were major constituents accounting for \u224874% of the total. Some prevalent non\u2010keratin constituents such as keratinocyte proline\u2010rich protein, loricrin and late envelope protein\u20107 were preferentially incorporated into envelopes. The results suggest a model where, as previously observed in hair shaft and nail plate, a diversity of cellular proteins are incorporated. They also help rationalize the minimal effect on epidermis of ablating genes for specific single envelope structural components. The quantitative profile of constituent proteins provides a foundation for future exploration of envelope perturbations that may occur in pathological conditions.<\/jats:p>","DOI":"10.1111\/exd.13925","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:19:10Z","timestamp":1553732350000},"page":"618-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Human stratum corneum proteomics reveals cross\u2010linking of a broad spectrum of proteins in cornified envelopes"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5699-6500","authenticated-orcid":false,"given":"Noreen","family":"Karim","sequence":"first","affiliation":[{"name":"Department of Biotechnology Medical Genetics Research Laboratory Quaid\u2010i\u2010Azam University Islamabad Pakistan"},{"name":"Department of Environmental Toxicology University of California Davis California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3870-3302","authenticated-orcid":false,"given":"Brett S.","family":"Phinney","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility University of California Davis California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Salemi","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility University of California Davis California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Wen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology University of California Davis California"},{"name":"Forensic Science Program University of California Davis California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-3085","authenticated-orcid":false,"given":"Muhammad","family":"Naeem","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Medical Genetics Research Laboratory Quaid\u2010i\u2010Azam University Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2058-4405","authenticated-orcid":false,"given":"Robert H.","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology University of California Davis California"},{"name":"Forensic Science Program University of California Davis California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)62353-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1619"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.09.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7824952"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0395-279"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1044"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.2.389"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.2.381"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200706187"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.01661.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00108"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.06.017"},{"key":"e_1_2_8_14_1","first-page":"229","volume":"62","author":"Rice R. 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