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However, the roles and the detailed mechanisms of H<jats:sub>2<\/jats:sub>S on skin wound healing are not known well. In the present study, 129S1\/SvImJ mice were intraperitoneally injected with NaHS (50\u00a0\u03bcmol\/kg\/d) for 2\u00a0weeks. Then, a round wound of 6\u00a0mm diameter with depth into the dermis was made. The skin wound area, blood perfusion, superoxide production, malondialdehyde (MDA) levels, total antioxidant capacity (T\u2010AOC), expression of vascular endothelial growth factor (VEGF), dynamin\u2010related protein 1 (DRP1) and optic atrophy 1 (OPA1) were measured. After NaHS (50\u00a0\u03bcmol\/L) pre\u2010administration for 4\u00a0hours, cell migration rate, DRP1, OPA1 and \u03b1\u2013smooth muscle actin (\u03b1\u2010SMA) expression, superoxide production and mitochondrial membrane potential in primary skin fibroblasts were measured. Tube formation in human umbilical vein endothelial cells (HUVECs) and cell migration in human keratinocytes were also measured. The results showed that NaHS pretreatment significantly accelerated wound healing and improved blood flow in the wound after operation. NaHS increased VEGF expression in the wound and promoted tube formation in HUVECs. Meanwhile, NaHS attenuated reactive oxygen species (ROS) production, suppressed MDA level but restored T\u2010AOC in the wound. NaHS also promoted skin fibroblasts migration and \u03b1\u2010SMA expression after scratch. Moreover, NaHS alleviated ROS, increased mitochondrial membrane potential, decreased DRP1 but enhanced OPA1 expression in skin fibroblasts after scratch. NaHS also accelerated human keratinocytes migration after scratch. Taken together, exogenous H<jats:sub>2<\/jats:sub>S supplementary accelerated the skin wound healing, which might be related to oxidative stress inhibition and VEGF enhancement.<\/jats:p>","DOI":"10.1111\/exd.13930","type":"journal-article","created":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T06:08:53Z","timestamp":1553926133000},"page":"776-785","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Exogenous hydrogen sulphide supplement accelerates skin wound healing via oxidative stress inhibition and vascular endothelial growth factor enhancement"],"prefix":"10.1111","volume":"28","author":[{"given":"Mengting","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Dermatology Affiliated Hospital of Nantong University Nantong China"},{"name":"Department of Pharmacology School of Pharmacy Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyun","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmacy Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Affiliated Hospital of Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmacy Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0569-8252","authenticated-orcid":false,"given":"Shengju","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Affiliated Hospital of Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,15]]},"reference":[{"key":"e_1_2_8_2_1","author":"Rousselle P.","year":"2018","journal-title":"Adv. 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