{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:48:07Z","timestamp":1769075287549,"version":"3.49.0"},"reference-count":16,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T00:00:00Z","timestamp":1562112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006162","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia do Estado de Pernambuco","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006162","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although several cytokines and chemokines have been investigated as possible mediators of fibrosis in systemic sclerosis (SSc), specific correlation between cytokines and organ involvement have not been found yet, and a cytokine profile characteristic of SSc is far to be identified. We studied the profile of antifibrotic and profibrotic transcripts involved in skin of SSc patients. The mRNA expression was detected by fluorescence\u2010based quantitative real\u2010time PCR (qPCR) in skin's biopsies from 14 patients with SSc and 5 healthy controls. PDGF\u2010A, CTGF, CCL3, IL\u20106, IL\u201013, IL\u20107, IFN\u03b3, IL\u201017, IL\u201022 and RORc were analysed in these samples. CCL3, IL\u20107, IL\u201013 and IFN\u2010\u03b3 were more expressed in skin's biopsy of patients with SSc (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0002, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0082, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0243, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0335, respectively) when compared with healthy controls. We also found a positive correlation between CCL3 and IL\u20107 transcripts (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0050 <jats:italic>r<\/jats:italic>\u00a0=\u00a00.7187). Furthermore, we observed that patients with lung involvement had lower expression of PDGF\u2010A (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0385). We found an increase in IL\u20107, IFN\u2010\u03b3, CCL3 and IL\u201013 relative mRNA expressions on the skin's biopsy of patients with SSc, and a positive correlation between IL\u20107 and CCL3. These molecules are involved in the pathogenesis of SSc, and how their interactions occur should be the subject of further studies.<\/jats:p>","DOI":"10.1111\/exd.13982","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T17:16:17Z","timestamp":1559668577000},"page":"1172-1175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CCL3, IL\u20107, IL\u201013 and IFN\u03b3 transcripts are increased in skin's biopsy of systemic sclerosis"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9014-6529","authenticated-orcid":false,"given":"Rafaela Silva Guimar\u00e3es","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Department of Rheumatology Clinical Hospital of Federal University of Pernambuco Recife Brazil"},{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelly Cristiny","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a Tavares","family":"Dantas","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Clinical Hospital of Federal University of Pernambuco Recife Brazil"},{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson Rodrigues de","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moacyr J. B. M.","family":"Rego","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emerson Andrade","family":"Lima","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Clinical Hospital of Federal University of Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan da Rocha","family":"Pitta","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela Luzia Branco Pinto","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Rheumatology Clinical Hospital of Federal University of Pernambuco Recife Brazil"},{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maira Galdino da Rocha","family":"Pitta","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunomodula\u00e7\u00e3o e Novas Abordagens Terap\u00eauticas N\u00facleo de Pesquisa em Inova\u00e7\u00e3o Terap\u00eautica Universidade Federal de Pernambuco Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00128-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2007.086850"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-015-0506-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.09.015"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400115200"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4334"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0214685"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.6.3088"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.00929.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.137349"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"6370","DOI":"10.4049\/jimmunol.151.11.6370","volume":"151","author":"Waal Malefyt R.","year":"1993","journal-title":"J. Immunol."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-015-0708-0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90021-3"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23451"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-14-80"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-258459"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.13982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.13982","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.13982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T09:34:53Z","timestamp":1694252093000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.13982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,3]]},"references-count":16,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/exd.13982"],"URL":"https:\/\/doi.org\/10.1111\/exd.13982","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,3]]},"assertion":[{"value":"2018-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}