{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T22:14:12Z","timestamp":1759529652695,"version":"3.40.5"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["STE 1208\/11-1, 14-1, TR156, SFB 1292"],"award-info":[{"award-number":["STE 1208\/11-1, 14-1, TR156, SFB 1292"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Humanized mice represent a suitable preclinical test system for example therapeutic interventions in various disease settings, including infections. Here, we intended to establish such system for cutaneous leishmaniasis by infecting T, B and NK cell\u2010deficient mice adoptively transferred with human peripheral blood mononuclear cells (PBMC). <jats:italic>L\u00a0major<\/jats:italic> infection led to the establishment of parasite lesions harbouring viable parasites and human T cells, but parasite elimination was not seen due to a species\u2010specific activity of T cell\u2010derived human IFN\u03b3. In addition, up to 50% of infected mice succumbed to severe graft\u2010versus\u2010host disease. In summary, even though long\u2010term disease outcome assessments are impossible, this model of humanized mice can be used for studying lesion development and generation of oligoclonal anti\u2010parasite human T cell responses in vivo.<\/jats:p>","DOI":"10.1111\/exd.13999","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T18:42:46Z","timestamp":1562006566000},"page":"1087-1090","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Humanized mice in cutaneous leishmaniasis\u2014Suitability analysis of human PBMC transfer into immunodeficient mice"],"prefix":"10.1111","volume":"28","author":[{"given":"Michael R.","family":"Fischer","sequence":"first","affiliation":[{"name":"Department of Dermatology University Medical Center, Johannes Gutenberg\u2010University of Mainz Mainz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anja 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