{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:06:08Z","timestamp":1747184768754,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T00:00:00Z","timestamp":1566172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008664","name":"Ono Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008664","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004088","name":"Kowa Life Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Differentiation and proliferation of keratinocyte are controlled by various signalling pathways. The epidermal growth factor receptor (EGFR) is known to be an important regulator of multiple epidermal functions. Inhibition of EGFR signalling disturbs keratinocyte proliferation, differentiation and migration. Previous studies have revealed that one of the EGFR downstream signalling molecules, phospholipase C\u03b31 (PLC\u03b31), regulates differentiation, proliferation and migration of keratinocytes in in vitro cell culture system. However, the role of PLC\u03b31 in the regulation of keratinocyte functions in animal epidermis remains unexplored. In this study, we generated keratinocyte\u2010specific PLC\u03b31 knockout (KO) mice (PLC\u03b31 cKO mice). Contrary to our expectations, loss of PLC\u03b31 did not affect differentiation, proliferation and migration of interfollicular keratinocytes. We further examined the role of PLC\u03b31 in irritant contact dermatitis (ICD), in which epidermal cells play a pivotal role. Upon irritant stimulation, PLC\u03b31 cKO mice showed exaggerated ICD responses. Further study revealed that epidermal loss of PLC\u03b31 induced sebaceous gland hyperplasia, indicating that PLC\u03b31 regulates homeostasis of one of the epidermal appendages. Taken together, our results indicate that, although PLC\u03b31 is dispensable in interfollicular keratinocyte for normal differentiation, proliferation and migration, it is required for normal ICD responses. Our results also indicate that PLC\u03b31 regulates homeostasis of sebaceous glands.<\/jats:p>","DOI":"10.1111\/exd.14009","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T08:02:08Z","timestamp":1563955328000},"page":"1051-1057","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phospholipase C\u03b31 is required for normal irritant contact dermatitis responses and sebaceous gland homeostasis"],"prefix":"10.1111","volume":"28","author":[{"given":"Takatsugu","family":"Fukuyama","sequence":"first","affiliation":[{"name":"Laboratory of Genome and Biosignals, School of Life Sciences Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0369-6993","authenticated-orcid":false,"given":"Yoshikazu","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Laboratory of Genome and Biosignals, School of Life Sciences Tokyo University of Pharmacy and Life Sciences Tokyo Japan"},{"name":"PRIME, Japan Agency for Medical Research and Development Tokyo Japan"},{"name":"Department of Applied Biological Science, Faculty of Science and Technology Tokyo University of Science Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Kanemaru","sequence":"additional","affiliation":[{"name":"Laboratory of Genome and Biosignals, School of Life Sciences Tokyo University of Pharmacy and Life Sciences Tokyo Japan"},{"name":"Department of Applied Biological Science, Faculty of Science and Technology Tokyo University of Science Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiho","family":"Toyoda","sequence":"additional","affiliation":[{"name":"Laboratory of Genome and Biosignals, School of Life Sciences Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun\u2010Jun","family":"Jang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Ulsan National Institute of Science and Technology Ulsan Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pann\u2010Ghill","family":"Suh","sequence":"additional","affiliation":[{"name":"School of Life Sciences Ulsan National Institute of Science and Technology Ulsan Korea"},{"name":"Korea Brain Reaseach Institute Daegu Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoko","family":"Fukami","sequence":"additional","affiliation":[{"name":"Laboratory of Genome and Biosignals, School of Life Sciences Tokyo University of Pharmacy and Life Sciences Tokyo Japan"},{"name":"AMED\u2010CREST, Japan Agency for Medical Research and Development Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/312315a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/334661a0"},{"key":"e_1_2_8_4_1","first-page":"315","volume":"161","author":"Nakamura Y.","year":"2017","journal-title":"J. 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