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Beside cell\u2010to\u2010cell contacts and release of cytokines, hormones and second messengers, cells communicate each other through the release of extracellular vesicles containing DNA, RNA, microRNAs and proteins. It has been reported the alteration of extracellular vesicles trafficking in several diseases, but there is scarce evidence of the involvement of extracellular vesicles trafficking in the pathogenesis of psoriasis. The main goal of the study was to characterize the release, the cargo content and the capacity to transfer bioactive molecules of extracellular vesicles produced by keratinocytes following recombinant IL\u201017A treatment if compared to untreated keratinocytes. A combined approach of standard ultracentrifugation, RNA isolation and real\u2010time RT\u2010PCR techniques was used to characterize extracellular vesicles cargo. Flow cytometry was used to quantitatively and qualitatively analyse extracellular vesicles and to evaluate cell\u2010to\u2010cell extracellular vesicles transfer. We report that the treatment of human keratinocytes with IL\u201017A significantly modifies the extracellular vesicles cargo and release. Vesicles from IL\u201017A\u2010treated cells display a specific pattern of mRNA which is undid by IL\u201017A neutralization. Extracellular vesicles are taken up by acceptor cells irrespective of their content but only those derived from IL\u201017A\u2010treated cells enable recipient cells to express psoriasis\u2010associated mRNA. The results imply a role of extracellular vesicles in amplifying the pro\u2010inflammatory cascade induced in keratinocyte by pro\u2010psoriatic cytokines.<\/jats:p>","DOI":"10.1111\/exd.14015","type":"journal-article","created":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T11:48:40Z","timestamp":1564746520000},"page":"1066-1073","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Interleukin\u201017A affects extracellular vesicles release and cargo in human keratinocytes"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8950-9780","authenticated-orcid":false,"given":"Giorgio","family":"Mangino","sequence":"first","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3862-9731","authenticated-orcid":false,"given":"Marco","family":"Iuliano","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Carlomagno","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6295-3574","authenticated-orcid":false,"given":"Nicoletta","family":"Bernardini","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"},{"name":"Dermatology Unit \u201cDaniele Innocenzi\u201d Fiorini Hospital Terracina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8468-0677","authenticated-orcid":false,"given":"Paolo","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5163-2698","authenticated-orcid":false,"given":"Maria Vincenza","family":"Chiantore","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Istituto Superiore di Sanit\u00e0 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4478-5404","authenticated-orcid":false,"given":"Nevena","family":"Skroza","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"},{"name":"Dermatology Unit \u201cDaniele Innocenzi\u201d Fiorini Hospital Terracina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-2916","authenticated-orcid":false,"given":"Antonella","family":"Calogero","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"},{"name":"Istituto Chirurgico Ortopedico Traumatologico ICOT Latina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6300-8697","authenticated-orcid":false,"given":"Concetta","family":"Potenza","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"},{"name":"Dermatology Unit \u201cDaniele Innocenzi\u201d Fiorini Hospital Terracina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7638-1001","authenticated-orcid":false,"given":"Giovanna","family":"Romeo","sequence":"additional","affiliation":[{"name":"Department of Medico\u2010Surgical Sciences and Biotechnologies Sapienza University of Rome Latina Italy"},{"name":"Institute of Molecular Biology and Pathology Consiglio Nazionale delle Ricerche Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,28]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"119","volume":"7","author":"Ni C.","year":"2014","journal-title":"Clin. 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