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Melanoma develops when unrepaired DNA damage causes to skin cells to multiply and form malignant tumors. The current therapy is limited by the highly ability of this disease to metastasize rapidly. Plumbagin is a naphthoquinone (5\u2010hydroxy\u20102\u2010methyl\u20101, 4\u2010naphthoquinone), isolated from the roots of medicinal plant <jats:italic>Plumbago zeylanica,<\/jats:italic> and it is widely present in <jats:italic>Lawsonia inermis L<\/jats:italic>. It has been shown that plumbagin has an\u00a0anti\u2010proliferative and anti\u2010invasive activities in various cancer cell lines; however, the anti\u2010cancer and anti\u2010metastatic effects of plumbagin are largely unknown against melanoma cells. In this study, we evaluated the effect of plumbagin on B16F10 murine melanoma cells . Plumbagin decreased B16F10 cell viability as well as the cell migration, adhesion, and invasion. The molecular mechanism was studied, and plumbagin downregulated genes relevant in MAPK pathway, matrix metalloproteinases (MMP's), and cell adhesion. Furthermore, plumbagin elevated the expression of apoptosis and tumors suppressor genes, and genes significant in reactive oxygen species (ROS) response. Taken together, our findings suggest that plumbagin has an anti\u2010invasion and anti\u2010metastasis effect on melanoma cancer cells by acting on MAPK pathway and its related genes.<\/jats:p>","DOI":"10.1111\/exd.14079","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T20:01:52Z","timestamp":1580760112000},"page":"427-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Elucidation of the effect of plumbagin on the metastatic potential of B16F10 murine melanoma cells via MAPK signalling pathway"],"prefix":"10.1111","volume":"29","author":[{"given":"Fatima\u2010Zahra","family":"Alem","sequence":"first","affiliation":[{"name":"Alliance for Research on the Mediterranean and North Africa (ARENA) University of Tsukuba Tsukuba City Japan"},{"name":"Laboratory of Human Pathologies Biology Department of Biology Faculty of Sciences, and Genomic Center of Human Pathologies Faculty of Medicine and Pharmacy Mohammed V University of Rabat Rabat Morocco"},{"name":"Chimie et Biologie des Membranes et Nanoobjets University of Bordeaux CNRS UMR 5248 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0607-6562","authenticated-orcid":false,"given":"Meriem","family":"Bejaoui","sequence":"additional","affiliation":[{"name":"Alliance for Research on the Mediterranean and North Africa (ARENA) University of Tsukuba Tsukuba City Japan"},{"name":"School of Integrative and Global Majors (SIGMA) University of Tsukuba Tsukuba city Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4338-3323","authenticated-orcid":false,"given":"Myra O.","family":"Villareal","sequence":"additional","affiliation":[{"name":"Alliance for Research on the Mediterranean and North Africa (ARENA) University of Tsukuba Tsukuba City Japan"},{"name":"School of Integrative and Global Majors (SIGMA) University of Tsukuba Tsukuba city Japan"},{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boutayna","family":"Rhourri\u2010Frih","sequence":"additional","affiliation":[{"name":"Alliance for Research on the Mediterranean and North Africa (ARENA) University of Tsukuba Tsukuba City Japan"},{"name":"Chimie et Biologie des Membranes et Nanoobjets University of Bordeaux CNRS UMR 5248 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1399-9541","authenticated-orcid":false,"given":"Hiroko","family":"Isoda","sequence":"additional","affiliation":[{"name":"Alliance for Research on the Mediterranean and North Africa (ARENA) University of Tsukuba Tsukuba City Japan"},{"name":"School of Integrative and Global Majors (SIGMA) University of Tsukuba Tsukuba city Japan"},{"name":"Faculty of Life and Environmental Sciences University of Tsukuba Tsukuba City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-018-9893-y"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3892-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15510"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1003466"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12213"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601910373795"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","unstructured":"B. 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