{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T06:18:18Z","timestamp":1766902698817,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673067"],"award-info":[{"award-number":["81673067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pemphigus vulgaris (PV) is a regulatory T cell (Treg)\u2010associated autoimmune disease. Treg cells maintain immunosuppression by expressing the signature transcription factor FOXP3. MicroRNAs (miRNAs) have frequently emerged as regulators in Treg\u2010mediated immunosuppression. We previously found that miR\u2010338\u20103p was overexpressed in the peripheral blood mononuclear cells of patients with PV. Herein, we explored the role of miR\u2010338\u20103p in Treg\u2010mediated immunosuppression by quantitative real\u2010time polymerase chain reaction, analysis of public microarray data, miRNA transfection, Western blotting, flow cytometry, and luciferase reporter assays. Increased expression of miR\u2010338\u20103p was detected in\u00a0CD4+\u00a0T cells of active PV patients compared with those in healthy controls. Moreover, the miR\u2010338\u20103p level was positively related to disease severity. Bioinformatics prediction revealed that Runt\u2010related transcription factor 1 (<jats:italic>RUNX1<\/jats:italic>), a gene activating <jats:italic>FOXP3<\/jats:italic> expression, was a putative target of miR\u2010338\u20103p. There was a reduction of <jats:italic>FOXP3<\/jats:italic> and <jats:italic>RUNX1\u00a0<\/jats:italic>expression in the CD4+\u00a0T cells of patients with PV, along with significant correlations with the level of miR\u2010338\u20103p. MiRNA transfection, mRNA and protein analysis, and luciferase reporter assays verified that miR\u2010338\u20103p attenuated <jats:italic>FOXP3<\/jats:italic> expression by targeting <jats:italic>RUNX1<\/jats:italic>. This study suggests that excessive expression of miR\u2010338\u20103p attenuates the expression of <jats:italic>FOXP3\u00a0<\/jats:italic>by targeting <jats:italic>RUNX1<\/jats:italic>, contributing to Treg dysfunction in PV.<\/jats:p>","DOI":"10.1111\/exd.14111","type":"journal-article","created":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T15:44:49Z","timestamp":1589039089000},"page":"623-629","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Increased expression of miR\u2010338\u20103p impairs Treg\u2010mediated immunosuppression in pemphigus vulgaris by targeting <i>RUNX1<\/i>"],"prefix":"10.1111","volume":"29","author":[{"given":"Meinian","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology Nanfang Hospital Southern Medical University Guangzhou 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