{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T08:06:59Z","timestamp":1788336419700,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006286","name":"Kongelig Hofbuntmager Aage Bangs Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several cancers, including mycosis fungoides (MF), have reported dysregulation of miR\u2010195\u20105p. miR\u2010195\u20105p plays a role in cell cycle regulation in several malignant diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to investigate: (a) the expression level of miR\u2010195\u20105p in lesional MF skin biopsies and (b) the potential regulatory roles of miR\u2010195\u20105p in MF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction (RT\u2010qPCR) was used to determine miR\u2010195\u20105p expression in MF skin biopsies and cell lines. The effect of miR\u2010195\u20105p and ADP\u2010ribosylation factor\u2010like protein 2 (ARL2) on cell cycle and apoptosis was measured by flow cytometry assays. Changes in ARL2 expression were determined by RT\u2010qPCR and Western blotting (WB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found lower expression levels of miR\u2010195\u20105p in lesional skin from MF patients compared with non\u2010lesional MF skin and skin from healthy volunteers. Additionally, miR\u2010195\u20105p showed lower expression levels in the skin from patients with disease progression compared with patients with stable disease. In vitro studies showed that overexpression of miR\u2010195\u20105p induced a cell cycle arrest in G0G1. Using microarray analysis, we identified several genes that were regulated after miR\u2010195\u20105p overexpression. The most downregulated gene after miR\u2010195\u20105p mimic transfection was ARL2. RT\u2010qPCR and WB analyses confirmed downregulation of ARL2 following transfection with miR\u2010195\u20105p mimic. Lastly, transfection with siRNA against ARL2 also induced a G0G1 arrest.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Upregulation of miR\u2010195\u20105p in MF inhibits cycle arrest by downregulation of ARL2. miR\u2010195\u20105p may thus function as a tumor suppressor in MF and low miR\u2010195\u20105p expression in lesional MF skin may promote disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/exd.14124","type":"journal-article","created":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T20:29:59Z","timestamp":1591216199000},"page":"1141-1149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Suppressed microRNA\u2010195\u20105p expression in mycosis fungoides promotes tumor cell proliferation"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-8671","authenticated-orcid":false,"given":"Anne 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