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Therefore, understanding the mitochondrial metabolic response to migration in the presence of ROS should be a key to understanding repigmentation in vitiligo. This study aimed to investigate the energy mechanism associated with the ROS\u2010mediated attenuation of melanocyte migration. After melanocytes were pretreated with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, their ATP production, migratory ability, ultrastructural changes and Mitochondrial Permeability Potential were analysed. The results showed that, in parallel with the decreased ATP production, the migratory ability of melanocytes was significantly inhibited by oxidative stress. Supplementation with exogenous ATP reversed the suppressed ATP\u2010dependent migration of melanocytes. Melanocytes were then stressed with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and Agilent Whole Human Genome microarray analysis identified 763 up\u2010regulated mRNAs and 1117 down\u2010regulated mRNAs. Among them, 11 of the encoded proteins were involved in mitochondrial ATP production and their expression levels were verified. The decreased expression of NADH dehydrogenase 2(ND<jats:sub>2)<\/jats:sub>, cytochrome c oxidase 1(COX1) and cytochrome c oxidase 3(COX3) was shown to be involved in the depletion of mitochondrial ATP production, which was coupled with the impaired migratory potential. These results indicate that the migration of melanocytes relies heavily on an inexhaustible supply of ATP from mitochondria.<\/jats:p>","DOI":"10.1111\/exd.14134","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T21:07:03Z","timestamp":1593032823000},"page":"733-741","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["H<sub>2<\/sub>O<sub>2<\/sub>\u2010induced oxidative stress disrupts mitochondrial functions and impairs migratory potential of human epidermal melanocytes"],"prefix":"10.1111","volume":"29","author":[{"given":"Ping","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Dermatology Affiliated Hospital of Nanjing University of Chinese Medicine  Nanjing China"},{"name":"First Clinical College Nanjing University of Chinese Medicine  Nanjing 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