{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:20:14Z","timestamp":1792668014938,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Skin models mimicking features of psoriasis\u2010related inflammation are needed to support the development of new drugs in dermatology. Reconstructed skin models lack tissue complexity, including a fully competent skin barrier, and presence and\/or diversity of immune cells. Here, we describe InflammaSkin\u00ae, a novel human Th17\u2010driven ex vivo skin inflammation model. In this model, skin\u2010resident T cells are in situ activated by intradermal injection of anti\u2010CD3 and anti\u2010CD28 antibodies and Th17 cell polarization is sustained by culture in a chemically defined medium supplemented with IL\u20101\u03b2, IL\u201023 and TGF\u2010\u03b2 for seven days. The acquired Th17 signature is demonstrated by the sustained secretion of IL\u201017A, IL\u201017AF, IL\u201017F, IL\u201022, IFN\u2010\u03b3, and to some degree IL\u201015 and TNF\u2010\u03b1 observed in the activated ex vivo skin inflammation model compared with the non\u2010activated skin model control. Furthermore, expression of S100A7 and Keratin\u201016 by keratinocytes and loss of epidermal structure integrity occur subsequently to in situ Th17cell activation, demonstrating cellular crosstalk between Th17 cells and keratinocytes. Finally, we demonstrate the use of this model to investigate the modulation of the IL\u201023\/IL\u201017 immune axis by topically applied anti\u2010inflammatory compounds. Taken together, we show that by in situ activation of skin\u2010resident Th17 cells, the InflammaSkin\u00ae model reproduces aspects of inflammatory responses observed in psoriatic lesions and could be used as a translational tool to assess efficacy of test compounds.<\/jats:p>","DOI":"10.1111\/exd.14160","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T09:19:49Z","timestamp":1596273589000},"page":"993-1003","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Development and characterization of a human Th17\u2010driven ex vivo skin inflammation model"],"prefix":"10.1111","volume":"29","author":[{"given":"Claire","family":"Jardet","sequence":"first","affiliation":[{"name":"Genoskin  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"David","sequence":"additional","affiliation":[{"name":"Genoskin  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Braun","sequence":"additional","affiliation":[{"name":"Genoskin  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Descargues","sequence":"additional","affiliation":[{"name":"Genoskin Inc.  Salem Massachussetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Louis","family":"Grolleau","sequence":"additional","affiliation":[{"name":"Service de Chirurgie Plastique et Reconstructrice CHU Toulouse Rangueil  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephine","family":"Hebsgaard","sequence":"additional","affiliation":[{"name":"Skin Research LEO Pharma A\/S  Ballerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Norsgaard","sequence":"additional","affiliation":[{"name":"Skin Research LEO Pharma A\/S  Ballerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4097-9634","authenticated-orcid":false,"given":"Paola","family":"Lovato","sequence":"additional","affiliation":[{"name":"Skin Research LEO Pharma A\/S  Ballerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.1994.5810077.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23435.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080173"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2008.07.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902464"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/718725"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.03.003"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2016.05.008"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.417"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/aivt.2014.0001"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00630"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09447"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1698"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(80)90047-X"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.09.029"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147979"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/mps2020045"},{"key":"e_1_2_8_20_1","first-page":"130","volume":"2010","author":"Gudjonsson J. 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