{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:20:00Z","timestamp":1762161600082,"version":"3.40.5"},"reference-count":68,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Psoriasis is a chronic inflammatory disorder with cutaneous and systemic manifestations and substantial negative effects on patients\u2019 quality of life. MicroRNAs (miRNAs) are post\u2010transcriptional regulators of gene expression that play a role in the pathogenesis of psoriasis. Previously studies, from others and by us, highlighted specific miRNAs that are dysregulated in psoriatic lesions. MicroRNA\u2010197\u20103p (miR\u2010197) expression is downregulated in psoriatic lesions compared to normal or uninvolved skin in patients with psoriasis. We have previously reported that miR\u2010197 could modulate IL\u201022 and IL\u201017 signalling in psoriasis. Herein, we identify additional biochemical targets of miR\u2010197 in psoriasis. We applied a transcriptome\u2010wide biochemical approach, Protein argonaute\u20102 photoactivatable ribonucleoside\u2010enhanced crosslinking and immunoprecipitation (Ago2 PAR\u2010CLIP), to search for new targets of miR\u2010197 in live keratinocytes, and validated its results using reporter assay and analysing by Western blot protein levels in cells overexpressing miR\u2010197. Ago2 PAR\u2010CLIP identified biochemical targets of miR\u2010197, including the alpha subunit of the IL\u20106 receptor (<jats:italic>IL6R<\/jats:italic>). This work provides evidence that <jats:italic>IL6R<\/jats:italic> in bona\u2010fide biochemical target of miR\u2010197. IL6R is known to be up\u2010regulated in psoriasis and even was considered as a possible therapeutic target. From the present data and our previous studies, it appears that miR\u2010197 is a major regulator of the interaction between immune system cells and keratinocytes.<\/jats:p>","DOI":"10.1111\/exd.14169","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T02:48:29Z","timestamp":1597200509000},"page":"1177-1186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>IL6R<\/i> is a target of miR\u2010197 in human keratinocytes"],"prefix":"10.1111","volume":"30","author":[{"given":"Moamen","family":"Masalha","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Cell Biology Center for Cancer Research and Department of Medicine C Sheba Medical Center Tel Hashomer Israel"},{"name":"Faculty of Medicine Sackler School of Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Devorah","family":"Gur\u2010Wahnon","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Transcriptomics Nephrology and Hypertension Services Hadassah\u2010Hebrew University Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tal","family":"Meningher","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology Center for Cancer Research and Department of Medicine C Sheba Medical Center Tel Hashomer Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iddo Z.","family":"Ben\u2010Dov","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Transcriptomics Nephrology and Hypertension Services Hadassah\u2010Hebrew University Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riad","family":"Kassem","sequence":"additional","affiliation":[{"name":"Department of Dermatology Sheba Medical Center Tel Hashomer Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yechezkel","family":"Sidi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology Center for Cancer Research and Department of Medicine C Sheba Medical Center Tel Hashomer Israel"},{"name":"Faculty of Medicine Sackler School of Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8738-1840","authenticated-orcid":false,"given":"Dror","family":"Avni","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology Center for Cancer Research and Department of Medicine C Sheba Medical Center Tel Hashomer Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.82"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920210793175895"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06642"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.69"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10568.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000610"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/JID.2015.409"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020916"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107467"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13520"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4172\/1745-7580.10000111"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2018.05.040"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1027"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.06.035"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.03.009"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.3.761"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90183-X"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10191.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.037"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.315"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/adt.2004.2.363"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-11-44"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-015-0338-9"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.08.006"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.10.007"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016050585"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-8-r79"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts167"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10191.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks596"},{"key":"e_1_2_8_33_1","first-page":"11","volume":"539","author":"Spitzer J.","year":"2014","journal-title":"Methods Enzymol."},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.182758.111"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002484"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08170"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.05.022"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/437301"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605298103"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.031"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.16.6367"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2009.09156.x"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.03.049"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.006"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2012.20.2.171"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1031440"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1177\/2058738418795940"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.22323.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13260"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17391"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp407"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.8.3114-3123.2006"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-7-13"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700480"},{"key":"e_1_2_8_55_1","first-page":"1789","volume":"39","author":"Adachi W.","year":"1998","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_8_56_1","first-page":"1624","volume":"58","author":"Santamaria I.","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2012.04618.x"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1999.00622.x"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2008.04.005"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb941"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90411-7"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90438-8"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3136546"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3153"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00371814"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12876121"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-205002"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.11.022"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10005.x"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fexd.14169","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14169","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.14169","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T02:03:14Z","timestamp":1693360994000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.14169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,21]]},"references-count":68,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1111\/exd.14169"],"URL":"https:\/\/doi.org\/10.1111\/exd.14169","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"type":"print","value":"0906-6705"},{"type":"electronic","value":"1600-0625"}],"subject":[],"published":{"date-parts":[[2020,9,21]]},"assertion":[{"value":"2020-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}