{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:44:32Z","timestamp":1790642672208,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T00:00:00Z","timestamp":1611360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Atopic Dermatitis (AD) is a common inflammatory skin disease characterized by skin and systemic inflammation, and barrier dysfunction. Herein, we investigate the proteomic profile of AD skin barrier to identify a unique signature with an easy\u2010performed sampling approach. We enrolled 8 moderate\u2010to\u2010severe AD patients and 8 age\u2010 and gender\u2010matched healthy controls. Swabs were obtained from non\u2010lesional skin of retroauricular area and antecubital fold. Peptide mixtures obtained through protein precipitation and in\u2010solution digestion were analysed using NanoLC\u2010MS\/MS. Label\u2010free quantification and statistical analysis were conducted in MaxQuant and Perseus. Bioinformatics analysis was performed using Gene Ontology and STRING. We identified 908 proteins and 35 differentially expressed proteins were selected (fold change 2, FDR\u00a0&lt;\u00a00.05). Particularly, AD skin showed downregulation of skin hydration factors, structural and epidermal proteins, abnormalities in protease\u2010proteasome complex and lipid metabolism profile. Imbalance of antioxidant and inflammatory processes, along with TDRD15 upregulation was also observed. Our result showed partial overlap with skin biopsy\/tape\u2010strips studies, showing the reliability of our sampling approach which could be an easier method of detection of hallmark barrier proteins in AD. Furthermore, we displayed a new differentially expressed set of proteins, not yet explored in AD which can have a potential role in AD pathomechanisms.<\/jats:p>","DOI":"10.1111\/exd.14276","type":"journal-article","created":{"date-parts":[[2021,1,4]],"date-time":"2021-01-04T18:59:19Z","timestamp":1609786759000},"page":"811-819","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Proteomic analysis from skin swabs reveals a new set of proteins identifying skin impairment in atopic dermatitis"],"prefix":"10.1111","volume":"30","author":[{"given":"Paola","family":"Morelli","sequence":"first","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Gaspari","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Gabriele","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Dastoli","sequence":"additional","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Bennardo","sequence":"additional","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Brandusa","family":"Pavel","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Mississippi, University MS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cataldo","family":"Patruno","sequence":"additional","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7948-8536","authenticated-orcid":false,"given":"Ester","family":"Del Duca","sequence":"additional","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven P.","family":"Nistic\u00f2","sequence":"additional","affiliation":[{"name":"Department of Health Science \u2018Magna Gr\u00e6cia\u2019 University of Catanzaro Catanzaro Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,23]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0001-z"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","DOI":"10.23736\/S0392-0488.20.06532-3","article-title":"Clinical phenotypes of atopic dermatitis of the adult","author":"Patruno C","year":"2020","journal-title":"G Ital Dermatol Venereol"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3928\/19382359-20200217-01"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13693"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16934"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.01.011"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/jdds.jdds_15_19"},{"issue":"1","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.23736\/S0392-0488.19.06522-2","article-title":"Optimizing a clinical guidance for diagnosis of atopic dermatitis in adults: joint recommendations of the Italian Society of Dermatology and Venereology (SIDeMaST), Italian Association of Hospital Dermatologists (ADOI), and Italian Society of Allergological, Occupational and Environmental Dermatology (SIDAPA)","volume":"155","author":"Patruno C","year":"2020","journal-title":"G Ital Dermatol Venereol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02727"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.03.1160"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001377"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.10.033"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05357-4"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.261"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2019.1646128"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2016.08.122"},{"key":"e_1_2_8_21_1","article-title":"S100 proteins as an important regulator of macrophage inflammation","volume":"8","author":"Xia C","year":"2017","journal-title":"Front Immunol"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.08.002"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.073304"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.16691"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2019.020501"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.11.017"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2012.01472.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201846612"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2010.08.003"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201300081"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-7-22"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2008.10.003"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.07.069"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b00101"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.02.035"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13532"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajme.2017.09.001"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-015-1605-8"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01012"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309817"},{"issue":"11","key":"e_1_2_8_41_1","first-page":"2150","article-title":"Prolactin\u2010induced protein (PIP)\u2010characterization and role in breast cancer progression","volume":"8","author":"Urbaniak A","year":"2018","journal-title":"Am J Cancer Res"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1022795418010088"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14465"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2019.1603199"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2018.03.009"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2016.00045"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2511-3"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.5606\/ArchRheumatol.2018.6595"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19010247"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2019.01.023"},{"key":"e_1_2_8_51_1","first-page":"8","volume-title":"Current problems in dermatology","author":"Smeden J","year":"2016"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2016.09.003"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.3109\/09687688.2012.693210"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.14-14580"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00236"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14276","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/exd.14276","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/exd.14276","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T13:52:36Z","timestamp":1693403556000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/exd.14276"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,23]]},"references-count":54,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/exd.14276"],"URL":"https:\/\/doi.org\/10.1111\/exd.14276","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,23]]},"assertion":[{"value":"2020-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}