{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T04:24:58Z","timestamp":1785039898648,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/12475\u20104"],"award-info":[{"award-number":["2017\/12475\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Methylisothiazolinone (MI) and Methylchloroisothiazolinone (MCI) are among the most common skin sensitizers, yet the immunological events that occur during MCI\/MI allergic contact dermatitis (ACD) are still poorly understood. Objectives: To analyse dendrocytes, macrophage subtypes and T cells in skin during the elicitation phase of MCI\/MI ACD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirteen patients with positive patch test reactions to MCI\/MI (ACD group) and 11 individuals with negative patch test results were selected. Skin biopsies were only performed at 48\u00a0hours of patch testing. Immunohistochemistry was conducted to assess T cells, dendrocytes (Factor XIIIa), M1 (p\u2010Stat1, CD68) and M2 (c\u2010Maf, CD163) macrophages. Transcriptional analyses were performed for cytokines and related factors, and further compared to atopic dermatitis samples (n=4). Immunofluorescence assays addressed T cells location, along with IL\u20104 or IL\u201013, within the skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MCI\/MI elicited dermal dendrocytes and macrophages, pronouncedly the M2\u00a0subtype. T cells, majorly CD4+ T cells, accumulated in the perivascular areas. Similarly, abundant IL\u20104 protein was detected in these areas. There was an upregulation of IL\u20104 and IL\u201013\u00a0mRNA expression, a mild increase in <jats:italic>IFNG<\/jats:italic> mRNA levels and a down\u2010regulation of <jats:italic>RORC<\/jats:italic> in the ACD group. Immunofluorescence revealed dermal clusters of T cells co\u2010localized with IL\u20104.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>M2\u00a0macrophages and Th2 cells participate in the immunopathogenesis of MCI\/MI ACD. Dermal dendrocytes and M2\u00a0macrophages may assist the formation of CD4+ T cells perivascular clusters. These findings render a mechanistic insight into the MCI\/MI reaction. Further analysis at different timepoints of patch testing is required to fully comprehend this ACD kinetics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/exd.14442","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T19:01:11Z","timestamp":1628276471000},"page":"191-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Perivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinone"],"prefix":"10.1111","volume":"31","author":[{"given":"Anang\u00e9lica R.","family":"Virgens","sequence":"first","affiliation":[{"name":"Laboratory of Investigation in Medicine, LIM\u201056 Department of Dermatology, Tropical Medicine Institute of S\u00e3o Paulo University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"},{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9349-1616","authenticated-orcid":false,"given":"Heliana F. O.","family":"Goes","sequence":"additional","affiliation":[{"name":"Laboratory of Investigation in Medicine, LIM\u201056 Department of Dermatology, Tropical Medicine Institute of S\u00e3o Paulo University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"},{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel C.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Investigation in Medicine, LIM\u201056 Department of Dermatology, Tropical Medicine Institute of S\u00e3o Paulo University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Julia","family":"Pietrobon","sequence":"additional","affiliation":[{"name":"Laboratory of Investigation in Medicine, LIM\u201056 Department of Dermatology, Tropical Medicine Institute of S\u00e3o Paulo University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Cl\u00e1udia","family":"C. C. Branco","sequence":"additional","affiliation":[{"name":"Laboratory of Investigation in Medicine, LIM\u201056 Department of Dermatology, Tropical Medicine Institute of S\u00e3o Paulo University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmim A. L.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Department of Pathology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiura V.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel L.","family":"Orfali","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of S\u00e3o Paulo Medical School S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Fernando F.","family":"da 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